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首页> 外文期刊>Antimicrobial agents and chemotherapy. >Characterization of a Novel Plasmid-Mediated Cephalosporinase (CMY-9) and Its Genetic Environment in an Escherichia coli Clinical Isolate.
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Characterization of a Novel Plasmid-Mediated Cephalosporinase (CMY-9) and Its Genetic Environment in an Escherichia coli Clinical Isolate.

机译：新型质粒介导的头孢菌素酶（CMY-9）的表征及其在大肠杆菌临床分离株中的遗传环境。

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An Escherichia coli strain, HKYM68, which showed resistance to broad-spectrum cephalosporins was isolated from a sputum specimen in Japan. The high-level resistance of the strain to ceftazidime, cefpirome, and moxalactam was carried by a self-transferable plasmid. The beta-lactamase gene responsible for the resistance was cloned and sequenced. The deduced amino acid sequence of this gene product, CMY-9, had a single amino acid substitution (E85D), the residue reported to be part of the recognition site for the R1 side chain of beta-lactams, compared with the amino acid sequence of CMY-8 and also had 78% identity with the amino acid sequence of CepH, a chromosomal cephalosporinase of Aeromonas hydrophila. A sul1-type class 1 integron containing an aacA1-orfG gene cassette was identified upstream of bla(CMY-9) and ended with a truncated 3' conserved segment. The following 2.1 kb was almost identical to the common region of integrons In6 and In7 and the integron of pSAL-1, except that orf513 encoding a putative transposase was identified instead of orf341 due to addition of a single nucleotide. bla(CMY-9) was closely located downstream of the end of the common region. These observations are indicative of the exogenous derivation of bla(CMY-9) from some environmental microorganisms such as aeromonads.
机译：从日本的痰标本中分离出一种对广谱头孢菌素具有抗性的大肠杆菌HKYM68。该菌株对头孢他啶，头孢吡肟和莫拉内酰胺的高水平抗性由自转移质粒携带。克隆和测序了负责抵抗的β-内酰胺酶基因。该基因产物CMY-9的推导氨基酸序列具有单个氨基酸取代（E85D），据报道该残基是β-内酰胺R1侧链识别位点的一部分，与该氨基酸序列相比与CMY-8的氨基酸序列相同，并且与亲水性气单胞菌的染色体头孢菌素酶CepH的氨基酸序列具有78％的同一性。在bla（CMY-9）上游发现了一个含有aacA1-orfG基因盒的sul1型1类整合子，并以3'保守节段终止。接下来的2.1 kb与整合素In6和In7以及pSAL-1整合子的共同区域几乎相同，不同之处在于，由于添加了单个核苷酸，鉴定出编码假定的转座酶的orf513代替了orf341。 bla（CMY-9）紧邻共同区域末端的下游。这些观察结果表明bla（CMY-9）是从某些环境微生物（如气单胞菌）中外生衍生的。

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