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首页> 外文期刊>Antimicrobial agents and chemotherapy. >Effects of enrofloxacin on porcine phagocytic function.
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Effects of enrofloxacin on porcine phagocytic function.

机译：恩诺沙星对猪吞噬功能的影响。

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The interaction between enrofloxacin and porcine phagocytes was studied with clinically relevant concentrations of enrofloxacin. Enrofloxacin accumulated in phagocytes, with cellular concentration/extracellular concentration ratios of 9 for polymorphonuclear leukocytes (PMNs) and 5 for alveolar macrophages (AMs). Cells with accumulated enrofloxacin brought into enrofloxacin-free medium released approximately 80% (AMs) to 90% (PMNs) of their enrofloxacin within the first 10 min, after which no further release was seen. Enrofloxacin affected neither the viability of PMNs and AMs nor the chemotaxis of PMNs at concentrations ranging from 0 to 10 microg/ml. Enrofloxacin (0.5 microg/ml) did not alter the capability of PMNs and AMs to phagocytize fluorescent microparticles or Actinobacillus pleuropneumoniae, Pasteurella multocida, and Staphylococcus aureus. Significant differences in intracellular killing were seen with enrofloxacin at 5x the MIC compared with that for controls not treated with enrofloxacin. PMNs killed all S. aureus isolates in 3 h with or without enrofloxacin. Intracellular S. aureus isolates in AMs were less susceptible than extracellular S. aureus isolates to the bactericidal effect of enrofloxacin. P. multocida was not phagocytosed by PMNs. AMs did not kill P. multocida, and similar intra- and extracellular reductions of P. multocida isolates by enrofloxacin were found. Intraphagocytic killing of A. pleuropneumoniae was significantly enhanced by enrofloxacin at 5x the MIC in both PMNs and AMs. AMs are very susceptible to the A. pleuropneumoniae cytotoxin. This suggests that in serologically naive pigs the enhancing effect of enrofloxacin on the bactericidal action of PMNs may have clinical relevance.
机译：用临床相关浓度的恩诺沙星研究了恩诺沙星与猪吞噬细胞之间的相互作用。恩诺沙星在吞噬细胞中积累，细胞浓度/细胞外浓度比对于多形核白细胞（PMN）为9，对肺泡巨噬细胞（AM）为5。在最初的10分钟内，将含有积累的恩诺沙星的细胞放入不含恩诺沙星的培养基中，释放出大约80％（AMs）至90％（PMN）的恩诺沙星。恩诺沙星在0至10微克/毫升的浓度范围内均不影响PMN和AM的生存力或PMN的趋化性。恩诺沙星（0.5微克/毫升）不会改变PMN和AMs吞噬荧光微粒或胸膜肺炎放线杆菌，多杀巴斯德氏菌和金黄色葡萄球菌的能力。与未用恩诺沙星治疗的对照组相比，恩诺沙星在MIC的5倍处观察到细胞内杀伤力的显着差异。在有或没有恩诺沙星的情况下，PMN在3小时内杀死了所有金黄色葡萄球菌。 AMs中的细胞内金黄色葡萄球菌分离物比细胞外金黄色葡萄球菌对恩诺沙星的杀菌作用更不敏感。 PMN不吞噬多杀性巴氏杆菌。 AMs不能杀死多杀性疟原虫，并且发现恩诺沙星对多杀性疟原虫分离株的细胞内和细胞外还原作用相似。恩诺沙星在PMN和AM中的MIC均是MIC的5倍时，吞噬胸膜肺炎链球菌的杀伤作用明显增强。 AM对胸膜肺炎链球菌细胞毒素非常敏感。这表明在血清学上纯真的猪中，恩诺沙星对PMN杀菌作用的增强作用可能与临床有关。

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