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首页> 外文期刊>Antimicrobial agents and chemotherapy. >Epidemiology of Nalidixic Acid Resistance and TEM-1- and TEM-52-Mediated Ampicillin Resistance of Shigella sonnei Isolates Obtained in Korea between 1980 and 2000.
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Epidemiology of Nalidixic Acid Resistance and TEM-1- and TEM-52-Mediated Ampicillin Resistance of Shigella sonnei Isolates Obtained in Korea between 1980 and 2000.

机译：1980年至2000年在韩国获得的痢疾志贺氏菌分离株耐萘啶酸的流行病学以及TEM-1-和TEM-52介导的氨苄青霉素耐药性的流行病学。

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摘要

The resistance to ampicillin and nalidixic acid in Shigella sonnei isolates obtained in Korea during the period 1998 to 2000 was characterized. Recently (J. Y. Oh, H. S. Yu, S. K. Kim, S. Y. Seol, D. T. Cho, and J. C. Lee, J. Clin. Microbiol. 41:421-423, 2003) ampicillin and nalidixic acid resistance was found in 49 and 70%, respectively, of the 67 S. sonnei isolates obtained during this period. We analyzed 138 S. sonnei isolates collected during the same period. Ampicillin and nalidixic acid resistance was found in 30 and 86% of the isolates, respectively. The ampicillin resistance was mediated by a TEM-1 beta-lactamase, and TEM-52 extended-spectrum beta-lactamase was identified in one sporadic S. sonnei isolate from 1999. bla(TEM-1) and bla(TEM-52) were located in conjugative R-plasmids. Tn3 was detected in 41% of the ampicillin-resistant isolates. The R-plasmids from the transconjugants that transferred resistance to ampicillin exhibited different restriction fragment length polymorphism patterns, and a bla(TEM-1) probe was hybridized with the different fragments. The nalidixic acid resistance was exclusively associated with an amino acid substitution, Ser83-->Leu (TCG-->TTG), in gyrA. These findings indicate that the genetically related S. sonnei strains readily acquire resistance to ampicillin, streptomycin, trimethoprim, and sulfamethoxazole but not nalidixic acid through conjugative R-plasmids from difference sources when confronted by antibiotic selective pressures.
机译：在1998年至2000年期间，对韩国获得的志贺氏菌分离株对氨苄西林和萘啶酸的耐药性进行了表征。最近（JY Oh，HS HS，SK Kim，SY Seol，DT Cho和JC Lee，J. Clin。Microbiol。41：421-423，2003）发现氨苄西林和萘啶酸的耐药性分别为49％和70％，在此期间获得的67株S. sonnei分离株。我们分析了同期收集的138株S. sonnei分离株。分别在30％和86％的分离物中发现了氨苄青霉素和萘啶酸的耐药性。氨苄青霉素耐药性是由TEM-1β-内酰胺酶介导的，并且在1999年的一株散发性索氏杆菌中发现了TEM-52广谱β-内酰胺酶。bla（TEM-1）和bla（TEM-52）分别为位于共轭R质粒中。在41％的氨苄青霉素耐药菌株中检测到Tn3。从转移结合剂向氨苄青霉素传递抗性的R质粒显示出不同的限制性片段长度多态性模式，并且bla（TEM-1）探针与不同的片段杂交。萘啶酸的抗性仅与gyrA中的氨基酸取代Ser83-> Leu（TCG-> TTG）有关。这些发现表明，与遗传相关的S. sonnei菌株在面对抗生素选择压力时，可以通过来自不同来源的共轭R质粒轻易获得对氨苄青霉素，链霉素，甲氧苄氨嘧啶和磺胺甲恶唑的抗性，而对萘啶酸没有抗性。

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