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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Presence of simian virus 40 (SV40) is not frequent in Swedish malignant mesotheliomas.
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Presence of simian virus 40 (SV40) is not frequent in Swedish malignant mesotheliomas.

机译:在瑞典恶性间皮瘤中,猿猴病毒40(SV40)的出现并不常见。

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摘要

Simian virus 40 (SV40), a contaminant of polio vaccines used in the United States and Europe between 1955 and 1963, has been detected with high frequency in human malignant mesotheliomas (MM). In Sweden, from 1958, due to production in Javanese macaque kidney cells and SV40 testing from 1961, only polio vaccine claimed to be free of SV40 has been used. Hence, we aimed to screen Swedish MM patients for the presence of SV40. MATERIALS AND METHODS: Forty-one paraffin-embedded pleural MM samples, obtained from patients born from 1893-1958, were examined for amplifiable DNA. Testable samples were thereafter evaluated for the presence of SV40 DNA by two types of polymerase chain reactions (PCR) followed by sequencing. RESULTS: SV40 could be confirmed by sequencing in only 3 of the 30 MM samples containing DNA that could be amplified by PCR. CONCLUSION: The presence of SV40 is not frequent in Swedish MM patients.
机译:猿猴病毒40(SV40)是1955年至1963年间在美国和欧洲使用的脊髓灰质炎疫苗的污染物,已在人类恶性间皮瘤(MM)中被高频率检测到。在瑞典,从1958年开始,由于爪哇猕猴肾细胞的生产和从1961年开始的SV40测试,仅使用了声称不含SV40的脊髓灰质炎疫苗。因此,我们旨在筛查瑞典MM患者是否存在SV40。材料与方法:对从1893-1958年出生的患者中收集的41个石蜡包埋的胸膜MM标本进行了扩增DNA的检测。此后,通过两种类型的聚合酶链反应(PCR),然后进行测序,评估了可测样品中SV40 DNA的存在。结果:SV40可以通过仅对30 MM样品中的3份进行测序来确认,这些样品中包含可以通过PCR扩增的DNA。结论:瑞典MM患者中不经常出现SV40。

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