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首页> 外文期刊>Antimicrobial agents and chemotherapy. >Different pathways to acquiring resistance genes illustrated by the recent evolution of IncW plasmids.
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Different pathways to acquiring resistance genes illustrated by the recent evolution of IncW plasmids.

机译：IncW质粒的最新进化说明了获得抗性基因的不同途径。

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摘要

DNA sequence analysis of five IncW plasmids (R388, pSa, R7K, pIE321, and pIE522) demonstrated that they share a considerable portion of their genomes and allowed us to define the IncW backbone. Among these plasmids, the backbone is stable and seems to have diverged recently, since the overall identity among its members is higher than 95%. The only gene in which significant variation was observed was trwA; the changes in the coding sequence correlated with parallel changes in the corresponding TrwA binding sites at oriT, suggesting a functional connection between both sets of changes. The present IncW plasmid diversity is shaped by the acquisition of antibiotic resistance genes as a consequence of the pressure exerted by antibiotic usage. Sequence comparisons pinpointed the insertion events that differentiated the five plasmids analyzed. Of greatest interest is that a single acquisition of a class I integron platform, into which different gene cassettes were later incorporated, gave rise to plasmids R388, pIE522, and pSa, while plasmids R7K and pIE321 do not contain the integron platform and arose in the antibiotic world because of the insertion of several antibiotic resistance transposons.
机译：对五个IncW质粒（R388，pSa，R7K，pIE321和pIE522）的DNA序列分析表明，它们共享其基因组的相当一部分，并允许我们定义IncW骨架。在这些质粒中，主链是稳定的，并且最近似乎有所不同，因为其成员之间的总体同一性高于95％。唯一观察到显着变异的基因是trwA。编码序列的变化与oriT处相应TrwA结合位点的平行变化相关，表明这两组变化之间存在功能联系。当前的IncW质粒多样性是由于抗生素使用所施加的压力而获得抗生素抗性基因而形成的。序列比较指出了区分五个被分析质粒的插入事件。最令人感兴趣的是，一次收购了一个I类整合子平台，随后并入了不同的基因盒，产生了质粒R388，pIE522和pSa，而质粒R7K和pIE321不包含整合子平台，并在质粒中出现。抗生素界因为插入了几种抗生素抗性转座子。

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