...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Antimicrobial agents and chemotherapy. >Assessment of clarithromycin susceptibility in strains belonging to the Mycobacterium abscessus group by erm(41) and rrl sequencing.
【24h】

Assessment of clarithromycin susceptibility in strains belonging to the Mycobacterium abscessus group by erm(41) and rrl sequencing.

机译：通过erm（41）和rrl测序评估脓肿分枝杆菌属菌株中的克拉霉素敏感性。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Clarithromycin was the drug of choice for Mycobacterium abscessus infections until inducible resistance due to erm(41) was described. Because M. abscessus was split into M. abscessus sensu stricto, Mycobacterium massiliense, and Mycobacterium bolletii, we looked for erm(41) in the three species and determined their clarithromycin susceptibility levels. Ninety strains were included: 87 clinical strains from cystic fibrosis patients (61%) and others (39%), representing 43 M. abscessus, 30 M. massiliense, and 14 M. bolletii strains identified on a molecular basis, and 3 reference strains. Clarithromycin and azithromycin MICs were determined by broth microdilution and Etest with a 14-day incubation period. Mutations in rrl (23S rRNA gene) known to confer acquired clarithromycin resistance were also sought. erm(41) was detected in all strains but with two deletions in all M. massiliense strains. These strains were indeed susceptible to clarithromycin (MIC(90) of 1 mug/ml) except for four strains with rrl mutations. M. abscessus strains harbored an intact erm(41) but had a T/C polymorphism at the 28th nucleotide: T28 strains (Trp10 codon) demonstrated inducible clarithromycin resistance (MIC(90) of >16 mug/ml), while C28 strains (Arg10) were susceptible (MIC(90) of 2 mug/ml) except for two strains with rrl mutations. M. bolletii strains had erm(41) sequences similar to the sequence of the T28 M. abscessus group, associated with inducible clarithromycin resistance (MIC(90) of >16 mug/ml). erm(41) sequences appeared species specific within the M. abscessus group and were fully concordant with clarithromycin susceptibility when erm(41) sequencing was associated with detection of rrl mutations. Clarithromycin-resistant strains, including the six rrl mutants, were more often isolated in cystic fibrosis patients, but this was not significantly associated with a previous treatment.
机译：克拉霉素是脓肿分枝杆菌感染的首选药物，直到描述了由于erm引起的可诱导的耐药性为止（41）。由于脓肿分枝杆菌分为严格的脓肿分枝杆菌，大众分枝杆菌和bolletii分枝杆菌，因此我们在这三个物种中寻找了erm（41），并确定了它们对克拉霉素的敏感性水平。包括90种菌株：来自囊性纤维化患者的87株临床菌株（61％）和其他（39％），分别代表43M脓肿，30M Massiliense和14M bolletii菌株，以及3株参考菌株。 。克拉霉素和阿奇霉素的MIC通过肉汤微量稀释和Etest测定，孵育时间为14天。还寻求了已知赋予获得性克拉霉素抗性的rrl（23S rRNA基因）突变。在所有菌株中均检测到erm（41），但在所有M. massiliense菌株中均缺失了两个。这些菌株的确对克拉霉素敏感（MIC（90）为1马克杯/毫升），除了四个带有rrl突变的菌株。脓肿分支杆菌菌株具有完整的erm（41），但在第28个核苷酸处具有T / C多态性：T28菌株（Trp10密码子）表现出可诱导的克拉霉素抗性（MIC（90）> 16马克/毫升），而C28菌株（ Arg10）除两种带有rrl突变的菌株外均易感（MIC（90）为2 mug / ml）。 bolletii菌株的erm（41）序列与脓肿T28组的序列相似，与可诱导的克拉霉素抗性相关（MIC（90）> 16 mug / ml）。 erm（41）序列出现在脓肿分支组内，且具有种特异性，并且当erm（41）测序与rrl突变检测相关时，与克拉霉素敏感性完全一致。包括六个rrl突变体在内的克拉霉素抗性菌株在囊性纤维化患者中更常见，但这与先前的治疗没有显着相关性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号