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首页> 外文期刊>Antimicrobial agents and chemotherapy. >Comparison of the antimicrobial effects of chlorine, silver ion, and tobramycin on biofilm.
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Comparison of the antimicrobial effects of chlorine, silver ion, and tobramycin on biofilm.

机译：氯，银离子和妥布霉素对生物膜的抗菌作用比较。

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The systematic understanding of how various antimicrobial agents are involved in controlling biofilms is essential in order to establish an effective strategy for biofilm control, since many antimicrobial agents are effective against planktonic cells but are ineffective when they are used against the same bacteria growing in a biofilm state. Three different antimicrobial agents (chlorine, silver, and tobramycin) and three different methods for the measurement of membrane integrity (plate counts, the measurement of respiratory activity with 5-cyano-2,3-ditolyl tetrazolium chloride [CTC] staining, and BacLight Live/Dead staining) were used along with confocal laser scanning microscopy (CLSM) and epifluorescence microscopy to examine the activities of the antimicrobials on biofilms in a comparative way. The three methods of determining the activities of the antimicrobials gave very different results for each antimicrobial agent. Among the three antimicrobials, tobramycin appeared to be the most effective in reducing the respiratory activity of biofilm cells, based upon CTC staining. In contrast, tobramycin-treated biofilm cells maintained their membrane integrity better than chlorine- or silver-treated ones, as evidenced by imaging by both CLSM and epifluorescence microscopy. Combined and sequential treatments with silver and tobramycin showed an enhanced antimicrobial efficiency of more than 200%, while the antimicrobial activity of either chlorine or tobramycin was antagonized when the agents were used in combination. This observation makes sense when the different oxidative reactivities of chlorine, silver, and tobramycin are considered.
机译：为了建立有效的生物膜控制策略，系统地了解各种抗微生物剂如何参与控制生物膜至关重要，因为许多抗微生物剂对浮游细胞有效，但当它们对在生物膜中生长的同一细菌使用时无效州。三种不同的抗菌剂（氯，银和妥布霉素）和三种不同的膜完整性测量方法（板数，5-氰基-2,3-二甲苯基四唑氯化物[CTC]染色和BacLight的呼吸活动测量）将活/死染色与共聚焦激光扫描显微镜（CLSM）和落射荧光显微镜一起使用，以比较的方式检查抗菌剂对生物膜的活性。确定每种抗菌剂的三种方法得出的每种抗菌剂的结果截然不同。根据CTC染色，在三种抗菌剂中，妥布霉素似乎在减少生物膜细胞的呼吸活性方面最有效。相比之下，妥布霉素处理过的生物膜细胞比氯或银处理过的生物膜细胞保持更好的膜完整性，这由CLSM和落射荧光显微镜成像所证明。银和妥布霉素的联合和相继处理显示出超过200％的增强抗菌效率，而当这些药剂组合使用时，氯或妥布霉素的抗菌活性均被拮抗。当考虑氯，银和妥布霉素的不同氧化反应性时，这种观察是有意义的。

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