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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Pituitary tumor-transforming gene (PTTG): a novel target for anti-tumor therapy.
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Pituitary tumor-transforming gene (PTTG): a novel target for anti-tumor therapy.

机译：垂体肿瘤转化基因（PTTG）：抗肿瘤治疗的新目标。

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BACKGROUND: The proto-oncogene pituitary tumor-transforming gene (PTTG) is described as abundantly over-expressed in a variety of neoplasms with impact on neovascularization and tumor invasiveness. Based on these findings, we raised the question as to whether PTTG is a target for an anti-tumor therapy. MATERIALS AND METHODS: To investigate the impact of PTTG depletion on the human cervical cancer cell line HELA-S3, we used antisense-oligodesoxynucleotides (antisense-ODNs) and subjected them to in vitro transfection experiments. The PTTG mRNA level was determined by RT-PCR (45 cycles) and PTTG protein levels were determined by Western blot experiments. The impact of PTTG depletion on cell growth was determined 24, 48 and 72 hours post transfection by the trypan blue exclusion method. RESULTS: We found an antisense-ODN effective in down-regulation of PTTG mRNA and protein level. Furthermore, transfection resulted in a significant growth inhibitory effect as well as an increased level of apoptotic figures. CONCLUSION: Our studies revealed an anti-tumor potential for this target in cancer therapy.
机译：背景：原癌基因垂体肿瘤转化基因（PTTG）被描述为在多种肿瘤中过度表达，对新血管形成和肿瘤侵袭性有影响。基于这些发现，我们提出了有关PTTG是否是抗肿瘤治疗靶点的问题。材料与方法：为了研究PTTG耗竭对人宫颈癌细胞株HELA-S3的影响，我们使用了反义寡脱氧核苷酸（antisense-ODNs）并将其进行了体外转染实验。通过RT-PCR（45个循环）确定PTTG mRNA水平，并通过Western印迹实验确定PTTG蛋白水平。通过锥虫蓝排除法在转染后24、48和72小时确定PTTG耗尽对细胞生长的影响。结果：我们发现了一种反义ODN，可有效下调PTTG mRNA和蛋白水平。此外，转染导致明显的生长抑制作用以及凋亡水平的升高。结论：我们的研究显示该靶标在癌症治疗中具有抗肿瘤潜力。

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