...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Human sarcoma cell lines MES-SA and MES-SA/Dx5 as a model for multidrug resistance modulators screening.
【24h】

Human sarcoma cell lines MES-SA and MES-SA/Dx5 as a model for multidrug resistance modulators screening.

机译：人肉瘤细胞系MES-SA和MES-SA / Dx5作为多药抗性调节剂筛选的模型。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The choice of cell lines for multidrug resistance (MDR) modulators screening may affect the results obtained. Screening is most often performed in model systems which employ cell lines derived from haematological malignancies. Cell lines originating from solid tumours are far less popular. In the present work, we aimed to test the usefulness of the drug-sensitive human sarcoma cell line MES-SA, and its multidrug-resistant counterpart MES-SA/Dx5, as a model system for modulators' anti-MDR potency evaluation. Overexpression of P-glycoprotein in the resistant but not in the sensitive cell line was confirmed by flow cytometry and confocal microscopy. Flow cytometry demonstrated that verapamil and trifluoperazine reduced MDR in MES-SA/Dx5 cells as assessed by the rhodamine 123 accumulation test. Both modulators also restored in MES-SA/Dx5 cells the drug accumulation pattern typical for sensitive cells, as judged by confocal microscopy. We conclude that the MES-SA and MES-SA/Dx5 cell line pair constitute a good model for MDR modulators study.
机译：用于多药耐药性（MDR）调节剂筛选的细胞系的选择可能会影响获得的结果。筛选最常在采用源自血液恶性肿瘤的细胞系的模型系统中进行。源自实体瘤的细胞系远不那么受欢迎。在目前的工作中，我们旨在测试对药物敏感的人肉瘤细胞系MES-SA及其多药抗性对应的MES-SA / Dx5作为调节剂抗MDR效能评估的模型系统的有效性。通过流式细胞术和共聚焦显微镜证实了P-糖蛋白在耐药细胞中过表达，但在敏感细胞系中没有过表达。流式细胞术表明，如若丹明123累积试验所评估，维拉帕米和三氟拉嗪可降低MES-SA / Dx5细胞中的MDR。通过共聚焦显微镜判断，这两种调节剂还在MES-SA / Dx5细胞中恢复了敏感细胞典型的药物积累模式。我们得出结论，MES-SA和MES-SA / Dx5细胞系对构成MDR调节剂研究的良好模型。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号