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首页> 外文期刊>Antimicrobial agents and chemotherapy. >Cefotaxime-resistant Enterobacteriaceae isolates from a hospital in Warsaw, Poland: identification of a new CTX-M-3 cefotaxime-hydrolyzing beta-lactamase that is closely related to the CTX-M-1/MEN-1 enzyme.
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Cefotaxime-resistant Enterobacteriaceae isolates from a hospital in Warsaw, Poland: identification of a new CTX-M-3 cefotaxime-hydrolyzing beta-lactamase that is closely related to the CTX-M-1/MEN-1 enzyme.

机译：耐头孢噻肟的肠杆菌科细菌来自波兰华沙一家医院：鉴定了一种新的CTX-M-3头孢噻肟水解β-内酰胺酶，该酶与CTX-M-1 / MEN-1酶密切相关。

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摘要

A group of cefotaxime-resistant Citrobacter freundii and Escherichia coli isolates were collected by a clinical laboratory in a hospital in Warsaw, Poland, in July 1996. Detailed analysis has shown that all of these produced a beta-lactamase (pI, 8.4) belonging to the CTX-M family, one of the minor extended-spectrum beta-lactamase families with a strong cefotaxime-hydrolyzing activity. Sequencing has revealed that C. freundii isolates produced a new CTX-M-3 enzyme which is very closely related to the CTX-M-1/MEN-1 beta-lactamase, sporadically identified in Europe over a period of 6 years. Amino acid sequences of these two beta-lactamases differ at four positions: Val77Ala, Asp114Asn, Ser140Ala, and Asn288Asp (the first amino acid of each pair refers to CTX-M-1/MEN-1 and second refers to CTX-M-3). The partial sequence of the E. coli CTX-M gene was identical to the corresponding region of bla(CTX-M-3), but a transconjugant of the E. coli isolate expressed higher levels of resistance to beta-lactams than did C. freundii transconjugants. These resistance differences correlated with differences in plasmid DNA restriction patterns. Our results suggest that CTX-M genes have been spread among different species of the family Enterobacteriaceae in the hospital and that the CTX-M-3-expressing C. freundii strain causing routine urinary tract infections has been maintained for a relatively long time in the hospital environment.
机译：1996年7月，由波兰华沙一家医院的临床实验室收集了一批耐头孢噻肟的弗氏柠檬酸杆菌和大肠杆菌分离株。详细分析表明，所有这些分离物均产生β-内酰胺酶（pI，8.4） CTX-M家族是具有强大头孢噻肟水解活性的次要广谱β-内酰胺酶家族之一。测序表明，弗氏梭状芽胞杆菌分离物产生了一种新的CTX-M-3酶，该酶与CTX-M-1 / MEN-1β-内酰胺酶密切相关，后者在欧洲在6年内偶发。这两个β-内酰胺酶的氨基酸序列在四个位置不同：Val77Ala，Asp114Asn，Ser140Ala和Asn288Asp（每对的第一个氨基酸是CTX-M-1 / MEN-1，第二个氨基酸是CTX-M-3 ）。大肠杆菌CTX-M基因的部分序列与bla（CTX-M-3）的相应区域相同，但与C相比，大肠杆菌分离株的转导结合体对β-内酰胺类的抗性更高。 freundii转导结合剂。这些抗性差异与质粒DNA限制模式的差异相关。我们的结果表明，CTX-M基因已经在医院的肠杆菌科的不同物种中传播，并且引起常规尿路感染的表达CTX-M-3的弗氏梭菌菌株在医院中维持了相当长的时间。医院环境。

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