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首页> 外文期刊>Antimicrobial agents and chemotherapy. >Blue dye and red light, a dynamic combination for prophylaxis and treatment of cutaneous Candida albicans infections in mice.
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Blue dye and red light, a dynamic combination for prophylaxis and treatment of cutaneous Candida albicans infections in mice.

机译：蓝色染料和红色光，一种预防和治疗小鼠皮肤白色念珠菌感染的动态组合。

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The objective of this study was to investigate photodynamic therapy (PDT), using blue dye and red light, for prophylaxis and treatment of cutaneous Candida albicans infections in mice. A mouse model of skin abrasion infected with C. albicans was developed by inoculating wounds measuring 1.2 cm by 1.2 cm with 10(6) or 10(7) CFU. The use of a luciferase-expressing strain of C. albicans allowed real-time monitoring of the extent of infection in mice noninvasively through bioluminescence imaging. The phenothiazinium salts toluidine blue O (TBO), methylene blue (MB), and new methylene blue (NMB) were compared as photosensitizers (PS) for the photodynamic inactivation of C. albicans in vitro. PDT in vivo was initiated either at 30 min or at 24 h after fungal inoculation to investigate the efficacies of PDT for both prophylaxis and treatment of infections. Light at 635 +/- 15 nm or 660 +/- 15 nm was delivered with a light dose of 78 J/cm(2) (for PDT at 30 min postinfection) or 120 J/cm(2) (for PDT at 24 h postinfection) in multiple exposures with bioluminescence imaging taking place after each exposure of light. In vitro studies showed that NMB was superior to TBO and MB as the PS in the photodynamic inactivation of C. albicans. The efficacy of PDT was related to the ratio of PS concentration to fungal cell density. PDT in vivo initiated either at 30 min or at 24 h postinfection significantly reduced C. albicans burden in the infected mouse skin abrasion wounds. These data suggest that PDT is a viable approach for prophylaxis and treatment of cutaneous C. albicans infections.
机译：这项研究的目的是研究使用蓝色染料和红色光的光动力疗法（PDT），以预防和治疗小鼠皮肤白色念珠菌感染。通过用10（6）或10（7）CFU接种1.2厘米x 1.2厘米的伤口，建立了感染白色念珠菌的小鼠皮肤擦伤模型。使用白色念珠菌的荧光素酶表达菌株可以通过生物发光成像实时监测小鼠的感染程度。比较了吩噻嗪鎓盐甲苯胺蓝O（TBO），亚甲基蓝（MB）和新亚甲基蓝（NMB）作为光敏剂（PS）的体外白色念珠菌的光动力学灭活。真菌接种后30分钟或24小时开始体内PDT，以研究PDT预防和治疗感染的功效。 635 +/- 15 nm或660 +/- 15 nm的光以78 J / cm（2）（对于感染后30分钟的PDT适用）或120 J / cm（2）（对于PDT在24感染后h）多次曝光，每次曝光后进行生物发光成像。体外研究表明，在白色念珠菌的光动力学灭活中，NMB优于TBO和MB作为PS。 PDT的功效与PS浓度与真菌细胞密度的比率有关。感染后30分钟或24小时在体内开始进行PDT，可显着降低感染的小鼠皮肤擦伤伤口中的白色念珠菌负担。这些数据表明，PDT是预防和治疗皮肤白色念珠菌感染的可行方法。

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