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首页> 外文期刊>Antimicrobial agents and chemotherapy. >Assessment of the effect of amphotericin B on the vitality of Candida albicans.
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Assessment of the effect of amphotericin B on the vitality of Candida albicans.

机译：评估两性霉素B对白色念珠菌活力的影响。

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摘要

The processes involved in cell death are complex, and individual techniques measure specific fractions of the total population. The interaction of Candida albicans with amphotericin B was measured with fluorescent probes with different cellular affinities. These were used to provide qualitative and quantitative information of physiological parameters which contribute to fungal cell viability. SYBR Green I and 5,(6)-carboxyfluorescein were used to assess membrane integrity, and bis-(1,3-dibutylbarbituric acid)trimethine oxonol and 3,3-dihexyloxacarbocyanine iodide were used to evaluate alterations in membrane potential. The fluorescent indicators were compared with replication competency, the conventional indicator of viability. By using these tools, the evaluation of the response of C. albicans to amphotericin B time-kill curves delineated four categories which may represent a continuum between alive and dead. The data showed that replication competency (CFU per milliliter) as determined by conventional antifungal susceptibility techniques provided only an estimate of inhibition. Interpretation of fluorescent staining characteristics indicated that C. albicans cells which were replication incompetent after exposure to greater than 0.5 microgram of amphotericin B per ml still maintained degrees of physiological function.
机译：细胞死亡所涉及的过程非常复杂，并且个别技术可以测量总人口中的特定部分。用具有不同细胞亲和力的荧光探针测量白色念珠菌与两性霉素B的相互作用。这些用于提供定性和定量信息的生理参数，有助于真菌细胞的生存能力。 SYBR Green I和5，（6）-羧基荧光素用于评估膜的完整性，而双-（1,3-二丁基巴比妥酸）三甲氧肟醇和3,3-二己基氧杂碳菁氰化物碘化物用于评估膜电位的变化。将荧光指示剂与复制能力（生存力的常规指示剂）进行比较。通过使用这些工具，对白色念珠菌对两性霉素B时间杀伤曲线的反应进行了评估，描绘出了四类，它们代表了生与死之间的连续性。数据显示，通过常规抗真菌药敏技术测定的复制能力（每毫升CFU）仅提供了抑制作用的估计值。荧光染色特性的解释表明，暴露于每毫升大于0.5微克的两性霉素B后复制能力不佳的白色念珠菌细胞仍保持生理功能。

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