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首页> 外文期刊>Antimicrobial agents and chemotherapy. >VanE, a new type of acquired glycopeptide resistance in Enterococcus faecalis BM4405.
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VanE, a new type of acquired glycopeptide resistance in Enterococcus faecalis BM4405.

机译：VanE，粪肠球菌BM4405中一种新型的获得性糖肽耐药性。

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摘要

Enterococcus faecalis BM4405 was resistant to low levels of vancomycin (MIC, 16 microg/ml) and was susceptible to teicoplanin (MIC, 0.5 microg/ml). No PCR product was obtained when the total DNA of this clinical isolate was used as a template with primers specific for glycopeptide resistance genes vanA, vanB, vanC, and vanD. However, a 604-bp PCR fragment was obtained when V1 and V2 degenerate primers were used and total DNA was digested with HindIII as a template. The product was cloned and sequenced. The deduced amino acid sequence had greater identity (55%) with VanC than with VanA (45%), VanB (43%), or VanD (44%). This was consistent with the fact that BM4405 synthesized peptidoglycan precursors that terminated in D-serine residues. After induction with vancomycin, weak D,D-dipeptidase and penicillin-insensitive D,D-carboxypeptidase activities were detected in cytoplasmic extracts of BM4405, whereas a serine racemase activity was found in the membrane preparation. This new type of acquired glycopeptide resistance was named VanE.
机译：粪肠球菌BM4405对低水平的万古霉素（MIC，16微克/毫升）有抵抗力，对替考拉宁（MIC，0.5微克/毫升）敏感。当此临床分离株的总DNA用作模板时，没有糖分抗性基因vanA，vanB，vanC和vanD的特异性引物，则无法获得PCR产物。但是，当使用V1和V2简并引物并以HindIII为模板消化总DNA时，获得了604-bp PCR片段。克隆产物并测序。推导的氨基酸序列与VanC的同源性（55％）比与VanA（45％），VanB（43％）或VanD（44％）更大。这与以下事实一致：BM4405合成了以D-丝氨酸残基终止的肽聚糖前体。万古霉素诱导后，在BM4405的细胞质提取物中检测到弱的D，D-二肽酶活性和对青霉素不敏感的D，D-羧肽酶活性，而在膜制剂中发现了丝氨酸消旋酶活性。这种新型的获得性糖肽耐药性称为VanE。

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