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首页> 外文期刊>Antimicrobial agents and chemotherapy. >Comparison of visual and spectrophotometric methods of broth microdilution MIC end point determination and evaluation of a sterol quantitation method for in vitro susceptibility testing of fluconazole and itraconazole against trailing and nontrailing
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Comparison of visual and spectrophotometric methods of broth microdilution MIC end point determination and evaluation of a sterol quantitation method for in vitro susceptibility testing of fluconazole and itraconazole against trailing and nontrailing

机译：肉汤微稀释液的视觉和分光光度法的比较MIC终点测定和固醇定量方法的评估，用于氟康唑和伊曲康唑的体外药敏试验

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Visual determination of MIC end points for azole antifungal agents can be complicated by the trailing growth phenomenon. To determine the incidence of trailing growth, we performed testing of in vitro susceptibility to fluconazole and itraconazole using the National Committee for Clinical Laboratory Standards broth microdilution M27-A reference procedure and 944 bloodstream isolates of seven Candida spp., obtained through active population-based surveillance between 1998 and 2000. Of 429 C. albicans isolates, 78 (18.2%) showed trailing growth at 48 h in tests with fluconazole, and 70 (16.3%) showed trailing in tests with itraconazole. Of 118 C. tropicalis isolates, 70 (59.3%) showed trailing growth in tests with fluconazole, and 35 (29.7%) showed trailing in tests with itraconazole. Trailing growth was not observed with any of the other five Candida spp. tested (C. dubliniensis, C. glabrata, C. krusei, C. lusitaniae, and C. parapsilosis). To confirm whether or not isolates that showed trailing growth in fluconazole and/or itraconazole were resistant in vitro to these agents, all isolates that showed trailing growth were retested by the sterol quantitation method, which measures cellular ergosterol content rather than growth inhibition after exposure to azoles. By this method, none of the trailing isolates was resistant in vitro to fluconazole or itraconazole. For both agents, a 24-h visual end point or a spectrophotometric end point of 50% reduction in growth relative to the growth control after 24 or 48 h of incubation correlated most closely with the result of sterol quantitation. Our results indicate that MIC results determined by either of these end point rules may be more predictive of in vivo outcome for isolates that give unclear visual end points at 48 h due to trailing growth.
机译：视觉上确定唑类抗真菌剂的MIC终点可能会因拖尾的生长现象而变得复杂。为了确定拖尾生长的发生率，我们使用美国国家临床实验室标准肉汤微量稀释M27-A参考程序和七个念珠菌属的944血流分离株进行了体外对氟康唑和伊曲康唑的敏感性试验，这些活性是通过基于活跃人群的在1998年至2000年期间进行了监测。在429株白色念珠菌分离物中，使用氟康唑的试验在48小时时显示出缓慢增长，而使用伊曲康唑的试验在48小时时显示出缓慢增长。在118株热带假丝酵母菌中，氟康唑试验显示有70个（59.3％）拖尾增长，伊曲康唑试验显示有35个（29.7％）拖尾。其他五个假丝酵母属均未观察到尾随的生长。测试（C. dubliniensis，C。glabrata，C。krusei，C。lusitaniae和C. parapsilosis）。为了确认氟康唑和/或伊曲康唑中尾随生长的分离株是否对这些药物具有体外抗性，所有显示尾随生长的分离株均通过固醇定量方法进行了重新测试，该方法测量的是细胞麦角固醇含量而不是暴露于接触后的生长抑制唑类通过这种方法，尾随的分离株在体外对氟康唑或伊曲康唑均无耐药性。对于两种试剂，在孵育24或48小时后，相对于生长对照，生长减少的24小时视觉终点或分光光度终点与固醇定量的结果最密切相关。我们的结果表明，通过这些终点规则中的任何一个确定的MIC结果，对于由于生长落后而在48 h时提供不清楚的视觉终点的分离株，可能更能预测其体内结果。

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