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首页> 外文期刊>Applied biochemistry and biotechnology, Part A. enzyme engineering and biotechnology >Pentavalent arsenate reductase activity in cytosolic fractions of Pseudomonas sp., isolated from arsenic-contaminated sites of Tezpur, Assam
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Pentavalent arsenate reductase activity in cytosolic fractions of Pseudomonas sp., isolated from arsenic-contaminated sites of Tezpur, Assam

机译：从阿萨姆邦特兹布尔的砷污染位点分离出的假单胞菌假单胞菌胞质部分中的五价砷酸盐还原酶活性

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摘要

Pentavalent arsenate reductase activity was localized and characterized in vitro in the cytosolic fraction of a newly isolated bacterial strain from arsenic-contaminated sites. The bacterium was gram negative, rod-shaped, nonmotile, non-spore-forming, and noncapsulated, and the strain was identified as Pseudomonas sp. DRBS1 following biochemical and molecular approaches. The strain Pseudomonas sp. DRBS1 exhibited enzymatic machinery for reduction of arsenate(V) to arsenite(III). The suspended culture of the bacterium reduced more than 97% of As(V) (40-100 mM) to As(III) in 48 h. The growth rate and total cellular yield decreased in the presence of higher concentration of arsenate. The suspended culture repeatedly reduced 10 mM As(V) within 5 h up to five consecutive inputs. The cell-free extracts reduced 86% of 100 μM As(V) in 40 min. The specific activity of arsenate reductase enzyme in the presence of 100 μM arsenate is 6.68 μmol/min per milligram protein. The arsenate reductase activity is maximum at 30 °C and at pH 5.2. The arsenate reductase activity increased in the presence of electron donors like citrate, glucose, and galactose and metal ions like Cd~(+2), Cu~(+2), Ca~(+2), and Fe~(+2). Selenate as an electron donor also supports the growth of strain DRBS1 and significantly increased the arsenate reduction.
机译：五价砷酸盐还原酶活性在体外被新分离的，被砷污染的细菌菌株的胞质部分定位和表征。该细菌是革兰氏阴性，杆状，不运动，不形成孢子，没有包囊的，该菌株被鉴定为假单胞菌。 DRBS1遵循生化和分子方法。假单胞菌菌株。 DRBS1展示了将砷酸盐（V）还原为亚砷酸盐（III）的酶机制。细菌的悬浮培养物在48小时内将97％以上的As（V）（40-100 mM）还原为As（III）。在较高浓度的砷酸盐存在下，生长速率和总细胞产量下降。悬浮培养物在5小时内反复降低10 mM As（V），直至连续5次输入。无细胞提取物可在40分钟内减少100％MAs（V）的86％。在存在100μM砷酸盐的情况下，砷酸盐还原酶的比活性为每毫克蛋白质6.68μmol/ min。砷酸还原酶活性在30°C和pH 5.2时最大。砷酸盐还原酶活性在柠檬酸，葡萄糖和半乳糖等电子给体以及Cd〜（+2），Cu〜（+2），Ca〜（+2）和Fe〜（+2）等金属离子存在下增加。硒酸盐作为电子供体也支持菌株DRBS1的生长，并显着增加了砷酸盐的还原。

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