...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied biochemistry and biotechnology, Part A. enzyme engineering and biotechnology >Gene Cloning and Characterization of a Novel Salt-Tolerant and Glucose-Enhanced β-Glucosidase from a Marine Streptomycete
【24h】

Gene Cloning and Characterization of a Novel Salt-Tolerant and Glucose-Enhanced β-Glucosidase from a Marine Streptomycete

机译：海洋链霉菌的新型耐盐和葡萄糖增强的β-葡萄糖苷酶的基因克隆和鉴定

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The gene BglNH encoding a β-glucosidase was cloned from a marine streptomycete. Sequence analysis revealed that BglNH encoded a 456-aa peptide with a calculated mass of 51 kDa. The deduced amino acid sequence of BglNH showed the highest identities of 61 % with known β-glucosidases and contained a catalytic domain which belonged to the glycoside hydrolase family 1. The gene BglNH was expressed in Escherichia coli and the recombinant enzyme (r-BglNH) was purified. The optimum pH and temperature of r-BglNH were pH?6.0 and 45 °C, respectively. The r-BglNH displayed the typical salt-tolerant and glucose-enhanced characteristics. Its activity was remarkably enhanced in the presence of 0.5 M NaCl (rose more than 1.6-fold) and 0.1 M glucose (rose more than 1.4-fold). Moreover, r-BglNH displayed good pH stability and metal tolerance. It remained stable after incubating with buffers from pH?4.0 to 10.0, and most metal ions had no significant inhibition on its activity. These properties indicate that r-BglNH is an ideal candidate for further research and industrial applications.
机译：从海洋链霉菌克隆了编码β-葡糖苷酶的基因BglNH。序列分析显示，BglNH编码一个456-aa肽，计算质量为51 kDa。推导的BglNH氨基酸序列与已知的β-葡萄糖苷酶的同一性最高，为61％，并包含一个属于糖苷水解酶家族1的催化域。基因BglNH在大肠杆菌和重组酶（r-BglNH）中表达被纯化。 r-BglNH的最佳pH和温度分别为6.0〜45℃。 r-BglNH具有典型的耐盐和葡萄糖增强特性。在0.5 M NaCl（上升超过1.6倍）和0.1 M葡萄糖（上升超过1.4倍）的存在下，其活性显着增强。而且，r-BglNH显示出良好的pH稳定性和金属耐受性。用pH≥4.0至10.0的缓冲液温育后仍保持稳定，大多数金属离子对其活性无明显抑制作用。这些性质表明，r-BglNH是进一步研究和工业应用的理想候选者。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号