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首页> 外文期刊>Journal of cellular physiology. >Effects of photobiomodulation combined with MSCs transplantation on the repair of spinal cord injury in rat
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Effects of photobiomodulation combined with MSCs transplantation on the repair of spinal cord injury in rat

机译：photobiomodulation结合msc的影响移植修复脊髓受伤的老鼠

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摘要

Stem cell transplantation has shown promising regenerative effects against neural injury, and photobiomodulation (PBM) can aid tissue recovery. This study aims to evaluate the therapeutic effect of human umbilical cord mesenchymal stem cells (hUCMSCs) and laser alone or combined on spinal cord injury (SCI). The animals were divided into SCI, hUCMSCs, laser treatment (LASER) and combination treatment (hUCMSCs + LASER) groups. Cell-enriched grafts of hUCMSCs (1 x 10~6 cells/ml) were injected at the site of antecedent trauma in SCI model rats. A 2 cm~2 damaged area was irradiated with 630 nm laser at 100 mW/cm~2 power for 20min. Locomotion was evaluated using Basso-Beattie-Bresnahan (BBB) scores, and neurofilament repair were monitored by histological staining and diffusion tensor imaging (DTI). First, after SCI, the motor function of each group was restored with different degrees, the combination treatment significantly increased the BBB scores compared to either monotherapy. In addition, Nissl bodies were more numerous, and the nerve fibers were longer and thicker in the combination treatment group. Consistent with this, the in situ expression of NF-200 and glial fibrillary acidic protein in the damaged area was the highest in the combination treatment group. Finally, DTI showed that the combination therapy optimally improved neurofilament structure and arrangement. These results may show that the combination of PBM and hUCMSCs transplantation is a feasible strategy for reducing secondary damage and promoting functional recovery following SCI.
机译：干细胞移植已经证明有前途对神经损伤再生的影响,photobiomodulation (PBM)可以援助组织恢复。本研究旨在评估治疗人类脐带间充质干细胞的影响细胞(hUCMSCs)和激光单独或组合脊髓损伤(SCI)。分为SCI、hUCMSCs、激光治疗(激光)和联合治疗(hUCMSCs +激光)组。x 10 ~ 6细胞/毫升)注射的网站先前的创伤在SCI大鼠模型。受损区域与630 nm激光辐照100 mW /厘米~ 2 20分钟。评估使用Basso-Beattie-Bresnahan (BBB)分数,和神经丝修复被监控通过组织学染色和扩散张量成像(DTI)。每组的功能恢复不同程度的联合治疗显著增加的BBB评分比较单一疗法。多,神经纤维吗长和厚的联合治疗组。nf - 200和胶质原纤维酸性的表情蛋白质在受损区域是最高的联合治疗组。显示,联合治疗优化改善神经丝结构和安排。这些结果可能显示的结合PBM和hUCMSCs移植是可行的减少二次伤害和策略促进SCI后功能恢复。

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