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首页> 外文期刊>Journal of cellular physiology. >PAX7 promotes CD49f-positive dairy goat spermatogonial stem cells' self-renewal
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PAX7 promotes CD49f-positive dairy goat spermatogonial stem cells' self-renewal

机译：PAX7促进CD49f-positive乳制品山羊精原干细胞的自我更新

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摘要

Spermatogenesis is a complex process that originates from and depends on the spermatogonial stem cells (SSCs). The number of SSCs is rare, which makes the separation and enrichment of SSCs difficult and inefficient. The transcription factor PAX7 maintains fertility in normal spermatogenesis in mice. However, for large animals, much less is known about the SSCs' self-renewal regulation, especially in dairy goats. We isolated and enriched the CD49f-positive and negative dairy goat testicular cells by magnetic-activated cell sorting strategies. The RNA- sequencing and experimental data revealed that cells with a high CD49f and PAX7 expression are undifferentiated spermatogonia in goat testis. Our findings indicated that ZBTB16 (PLZF), PAX7, UN28A, BMPR1B, FGFR1, and FOXO1 were expressed higher in CD49f-positive cells as compared to negative cells and goat fibroblasts cells. The expression and distribution of PAX7 in dairy goat also have been detected, which gradually decreased in testis tissue along with the increasing age. When the PAX7 gene was overexpressed in dairy goat immortal mGSCs-l-SB germ cell lines, the expression of PLZF, GFRalpha1, ID4, and OCT4 was upregulated. Together, our data demonstrated that there is a subset of spermatogonial stem cells with a high expression of PAX7 among the CD49f~+ spermatogonia, and PAX7 can maintain the self-renewal of CD49f-positive SSCs.
机译：精子发生是一个复杂的过程源于,取决于精原干细胞(精原细胞)。这使得细胞的分离和浓缩困难和低效。因素PAX7保持生育能力正常在小鼠精子发生。动物细胞的了解要少得多自我更新的规定,特别是乳制品山羊。CD49f-positive和消极的乳制品山羊的睾丸细胞通过magnetic-activated细胞排序策略。数据显示,高CD49f和细胞PAX7表达式是无差别的spermatogonia在山羊的睾丸。表明ZBTB16 (PLZF)、PAX7 UN28A,BMPR1B、FGFR1、FOXO1高表达CD49f-positive细胞比消极的山羊细胞和成纤维细胞的细胞。和分销的PAX7乳制品山羊也被探测到,它逐渐减少睾丸组织随着年龄增加。PAX7基因过表达在乳制品山羊不朽的mGSCs-l-SB生殖细胞系,ID4 PLZF, GFRalpha1, OCT4调节。精原干细胞的一个子集高表达的PAX7 CD49f ~ +spermatogonia, PAX7可以维持的CD49f-positive精原细胞的自我更新。

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