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首页> 外文期刊>Journal of cellular physiology. >Enhanced protection against lipopolysaccharide-induced acute lung injury by autologous transplantation of adipose-derived stromal cells combined with low tidal volume ventilation in rats
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Enhanced protection against lipopolysaccharide-induced acute lung injury by autologous transplantation of adipose-derived stromal cells combined with low tidal volume ventilation in rats

机译：加强保护lipopolysaccharide-induced急性肺损伤自体脂肪移植的基质细胞结合低潮汐卷通风的老鼠

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摘要

Adipose-derived stromal cells (ADSCs) showed excellent capacity in regeneration and tissue protection. Low tidal volume ventilation (LVT) strategy demonstrates a therapeutic benefit on the treatment of acute lung injury/acute respiratory distress syndrome (ALI/ARDS). This study, therefore, aimed to undertaken determine whether the combined LVT and ADSCs treatment exerts additional protection against lipopolysaccharide (LPS)-induced ALI in rats. The animals were randomized into seven groups: Group I (control), Group II (instillation of LPS at 10 mg/kg in-tratracheally), Group III (LPS+LVT 6 ml/kg), Group IV (LPS+intravenous autologous 5 x 10~6 ADSCs which were pretreated with a scrambled small interfering RNA [siRNA] of keratinocyte growth factor [KGF] negative control), Group V (LPS+ADSCs which were pretreated with a scrambled siRNA of KGF, Group VI (LPS+LVT and ADSCs as in the Group IV), and Group VII (LPS+LVT and ADSCs as in the Group V). We found that levels of tumor necrosis factor-alpha, transforming growth factor-beta1, and interleukin (IL)-1beta and IL-6, the proinflammatory cytokines, were remarkably increased in LPS rats. Moreover, the expressions of ENaC, activity of Na, K-ATPase, and alveolar fluid clearance (AFC) were obviously reduced by LPS-induced ALI. The rats treated by ADSCs showed improved effects in all these changes of ALI and further enhanced by ADSCs combined with LVT treatment. Importantly, the treatment of ADSCs with siRNA-mediated knockdown of KGF partially eliminated the therapeutic effects. In conclusion, combined treatment with ADSCs and LVT not only is superior to either ADSCs or LVT therapy alone in the prevention of ALI. Evidence of the beneficial effect may be partly due to improving AFC by paracrine or systemic production of KGF and anti-inflammatory properties.
机译：脂肪基质细胞(ADSCs)显示优秀的能力和组织再生保护。战略演示了一个治疗中获益急性肺损伤/急性的治疗呼吸窘迫综合征(ALI / ARDS)。因此,研究旨在进行确定是否LVT ADSCs治疗相结合产生额外的保护全身的脂多糖(LPS)阿里的老鼠。动物随机分为7组:组我(控制),第二组(滴剂LPS 10毫克/公斤in-tratracheally),第三组(有限合伙人+ LVT 6毫升/公斤),第四组(有限合伙人+静脉注射自体5 x10 ~ 6 ADSCs使用一个炒角化细胞的小干扰RNA (siRNA)生长因子(KGF) -控制),V(有限合伙人+ ADSCs使用一个炒KGF核,第六组(有限合伙人+ LVT和ADSCs第四组)和第七组(有限合伙人+ LVT ADSCs在V)。我们发现水平的肿瘤坏死因子α,将增长factor-beta1,白介素(IL) 1β促炎细胞因子il - 6,显著增加了有限合伙人老鼠。博的表达、活动的Na、K-ATPase和肺泡液体间隙明显(亚)减少LPS-induced阿里。在所有这些ADSCs显示改进的影响由ADSCs阿里的变化,进一步增强结合从而可以治疗。治疗ADSCs siRNA-mediated击倒KGF部分消除了治疗效果。ADSCs和LVT不仅优于ADSCs或LVT治疗预防阿里。部分原因是亚足联通过旁分泌或改善系统性的生产KGF和消炎属性。

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