...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of cellular physiology. >MiR-4282 inhibits tumor progression through down-regulation of ZBTB2 by targeting LIN28B in oral squamous cell carcinoma
【24h】

MiR-4282 inhibits tumor progression through down-regulation of ZBTB2 by targeting LIN28B in oral squamous cell carcinoma

机译：mir - 4282抑制肿瘤恶化针对基因LIN28B ZBTB2的下调口腔鳞状细胞癌

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Oral squamous cell carcinoma (OSCC) is the most aggressive type of head and neck cancer with an unsatisfactory 5-year survival rate. MicroRNAs are a group of small noncoding RNAs reported to serve important roles in carcinogenesis, inhibiting certain gene expression via targeting the 3'-untranslated region of messenger RNAs (mRNAs). MiR-4282 has been newly discovered to be a tumor suppressor in colorectal cancer, but it has never been studied in OSCC. The present study aimed to uncover the role of miR-4282 in OSCC. We first confirmed that miR-4282 was downregulated in OSCC and validated its prognostic significance. Through gain-of -function assays, miR-4282 was discovered to inhibit proliferation, migration, and epithelial-to-mesenchymal transition, and induce apoptosis. By mechanistic research, we predicted via bioinformatics tools and confirmed by luciferase reporter and pulldown assays that miR-4282 targeted LIN28B, an RNA-binding protein, which has been reported to regulate RNA stability in cancers. Furthermore, we confirmed the interaction between LIN28B and zinc finger and BTB domain containing 2 (ZBTB2), and validated that miR-4282 regulated mRNA stability of ZBTB2 by inhibiting LIN28B. Rescue assays proved that miR-4282 inhibited tumor progression through LIN28B/ZBTB2 axis. In vivo assays proved that miR-4282 inhibited tumor growth in OSCC. In conclusion, the present study revealed that miR-4282 inhibited tumor progression through downregulation of ZBTB2 by targeting LIN28B in OSCC cells, indicating miR-4282 as a novel biomarker for OSC.
机译：口腔鳞状细胞癌(OSCC)是最咄咄逼人的头部和颈部癌症的类型不令人满意的5年生存率。据报道,一群小型非编码rna吗服务发生过程中重要的角色,通过针对抑制特定基因的表达信使rna的3 '非翻译区(mrna)。在结直肠癌肿瘤抑制基因,但它从来没有在OSCC学习。旨在发现mir - 4282在OSCC的角色。第一次证实,mir - 4282表达下调在OSCC和验证其预后的意义。发现mir - 4282抑制增殖,移民和epithelial-to-mesenchymal过渡,并诱导细胞凋亡。通过生物信息学的研究,我们预测工具经荧光素酶的记者和下拉分析目标基因LIN28B mir - 4282,rna结合蛋白,据报道调节RNA稳定癌症。我们证实了基因LIN28B和之间的交互锌指和BTB域包含2 (ZBTB2),和验证,mir - 4282规范mRNAZBTB2通过抑制基因LIN28B的稳定。化验证明,mir - 4282抑制肿瘤进程通过基因LIN28B / ZBTB2轴。化验证明,mir - 4282抑制肿瘤OSCC的增长。透露,mir - 4282抑制肿瘤ZBTB2的差别通过对这些发展针对基因LIN28B在OSCC细胞,指示mir - 4282作为OSC的一种新的生物标志物。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号