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首页> 外文期刊>Journal of cellular physiology. >Medial epithelial seam cell migration during palatal fusion
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Medial epithelial seam cell migration during palatal fusion

机译：seam内侧上皮细胞迁移期间腭的融合

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The mammalian secondary palate forms from two shelves of mesenchyme sheathed in a single-layered epithelium. These shelves meet during embryogenesis to form the midline epithelial seam (MES). Failure of MES degradation prevents mesenchymal confluence and results in a cleft palate. Previous studies indicated that MES cells undergo features of epithelial-to-mesenchymal transition (EMT) and may become migratory as part of the fusion mechanism. To detect MES cell movement over the course of fusion, we imaged the midline of fusing embryonic ephrin-B2/GFP mouse palates in real time using two-photon microscopy. These mice express an ephrin-B2-driven green fluorescent protein (GFP) that labels the palatal epithelium nuclei and persists in those cells through the time window necessary for fusion. We observed collective migration of MES cells toward the oral surface of the palatal shelf over 48 hr of imaging, and we confirmed histologically that the imaged palates had fused by the end of the imaged period. We previously reported that ephrin reverse signaling in the MES is required for palatal fusion. We therefore added recombinant EphA4/Fc protein to block this signaling in imaged palates. The blockage inhibited fusion, as expected, but did not change the observed migration of GFP-labeled cells. Thus, we uncoupled migration and fusion. Our data reveal that palatal MES cells undergo a collective, unidirectional movement during palatal fusion and that ephrin reverse signaling, though required for fusion, controls aspects of the fusion mechanism independent of migration.
机译：哺乳动物次级味觉从两种形式货架的间质护套单层上皮。在胚胎形成中线上皮细胞层(MES)。防止间叶细胞融合和结果腭裂。细胞进行的特性epithelial-to-mesenchymal (EMT)和过渡可能成为迁徙融合的一部分机制。融合过程中,融合成像中线胚胎ephrin-B2 / GFP小鼠的味觉在现实时间使用双光子显微镜。表达一个ephrin-B2-driven绿色荧光蛋白(GFP)标签腭上皮核和坚持这些细胞核聚变所需时间窗口。MES的集体迁移细胞向口腔腭货架表面超过48小时成像,并经病理证实成像的味觉年底融合了成像时期。MES需要反向信号腭的融合。EphA4 / Fc蛋白阻止这个信号成像的味觉。预期,但改变不了GFP-labeled细胞的迁移。非耦合迁移和融合。腭MES细胞接受一个集体,在腭融合和单向运动ephrin反向信号,虽然要求融合,融合的控制方面独立的迁移机制。

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  • 1. Cell fusion chiyanba [P] . 外国专利： JPH0691812B2 . 1994-11-16

    机译：cell fusion吃盐巴

  • 2. Cell fusion chiyanba [P] . 外国专利： JPH0653061B2 . 1994-07-20

    机译：cell fusion吃盐巴

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