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首页> 外文期刊>Journal of cellular physiology. >Gene expression and methylation profiles identified CXCL3 and CXCL8 as key genes for diagnosis and prognosis of colon adenocarcinoma
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Gene expression and methylation profiles identified CXCL3 and CXCL8 as key genes for diagnosis and prognosis of colon adenocarcinoma

机译：基因表达和甲基化配置文件确定CXCL3和CXCL8关键基因结肠腺癌的诊断和预后

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摘要

Colon adenocarcinoma (COAD) is one of the most common malignant tumors with high morbidity and mortality rates worldwide. Due to the poor clinical outcomes, it is indispensable to investigate novel biomarkers for the diagnosis and prognosis of COAD. The aim of this study is to explore key genes as potential biomarkers for the diagnosis and prognosis of COAD for clinical utility. Gene expression profiles (GSE44076 and GSE44861) and gene methylation profile (GSE29490) were analyzed to identify the aberrantly methylated-differentially expressed genes by R language and Perl software. Function enrichments were performed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway. Moreover, hub genes were identified through protein-protein interaction (PPI) network. Besides, key genes were found by the module analysis and The Cancer Genome Atlas (TCGA) survival analysis. Finally, TCGA data and quantitative real-time polymerase chain reaction (RT-qPCR) was used to validate the key genes involved in COAD. Our study found two hypomethylation-high-expression genes [CXCL3 and CXCL8) in COAD tissues compared with the adjacent normal tissues. These results were also confirmed by RT-qPCR with 25 pairs of COAD and adjacent normal tissues. Meanwhile, low expression of the two genes was associated with poor survival in patients with COAD. CXCL3 and CXCL8 may serve as key genes in the diagnosis and prognosis for COAD.
机译：结肠腺癌(COAD)是一种最与高发病率和常见的恶性肿瘤全球死亡率。临床结果,它是必不可少的研究新型生物标志物的诊断COAD和预后。探索关键基因的潜在生物标志物COAD为临床的诊断和预后实用程序。GSE44861)和基因甲基化谱(GSE29490)分析来识别异常methylated-differentially R的表达基因Perl语言和软件。是由基因本体论(去)和《京都议定书》吗百科全书的基因和基因组(KEGG)通路。此外,中心基因被确定通过蛋白质相互作用网络(PPI)。此外,模块关键基因被发现的分析和癌症基因组图谱(TCGA)生存分析。定量实时聚合酶链反应(RT-qPCR)是用于验证的关键基因参与COAD。hypomethylation-high-expression [CXCL3和基因CXCL8) COAD组织与邻边正常组织。通过RT-qPCR 25双COAD和相邻正常组织。两个基因与贫穷的生存COAD患者。关键基因的诊断和预后

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