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首页> 外文期刊>Journal of cellular physiology. >miR-188-5p suppresses cellular proliferation and migration via IL6ST: A potential noninvasive diagnostic biomarker for breast cancer
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miR-188-5p suppresses cellular proliferation and migration via IL6ST: A potential noninvasive diagnostic biomarker for breast cancer

机译：mir - 188 - 5 - p细胞抑制增殖及通过IL6ST迁移:一个潜在的非侵入性诊断为乳腺癌生物标记

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Previously, serum miR-188-5p is differentially expressed in breast cancer, but the diagnostic potential of circulating miR-188-5p as well as its regulatory mechanism in breast cancer remain uncertain. Herein, serum miR-188-5p was detected by real-time polymerase chain reaction in patients with breast cancer, breast fibroadenoma, and healthy subjects. Circulating miR-188-5p was abnormally elevated in patients with breast cancer as compared with these other two groups, and was reduced in patients with breast cancer following surgical treatment. Increased serum miR-188-5p corresponded to lymph node metastasis status and TNM stages of breast cancer. A receiver operating characteristic curve analysis of the ability to circulate miR-188-5p to distinguish between patients with breast cancer and either noncancerous patients or patients with breast fibroadenoma yielded corresponding areas under the curve of 0.894 and 8.814. miR-188-5p was downregulated in the highly malignant cancer line MDA-MB-231 relative to the less malignant MCF-7 cells. In vitro, functional analyses conducted via transfecting cells with mimics and inhibitors revealed miR-188-5p to suppress breast cancer cell proliferation and migration, which was mediated by its downstream target IL6ST. Comparison of intracellular and exosomal miR-188-5p levels indicated that miR-188-5p was selectively sorted into exosomes derived from MDA-MB-231 cells rather than those from MCF-7 cells. However, exosomal miR-188-5p levels in the serum of patients with breast cancer were reduced compared to healthy controls and did not differ relative to patients with breast fibroadenoma. In summary, miR-188-5p acts in a tumor-suppressive manner in breast cancer progression and may serve as a noninvasive early diagnostic biomarker and therapeutic target in breast cancer.
机译：此前,血清mir - 188 - 5 - p是不同的表达在乳腺癌,但诊断潜在的循环mir - 188 - 5 - p和其监管机制在乳腺癌仍然存在不确定的。通过实时聚合酶链反应乳腺癌患者,乳房纤维腺瘤,和健康的话题。乳腺癌患者的异常升高癌症与其他两组相比,降低乳腺癌患者手术治疗。mir - 188 - 5 - p与淋巴结转移地位和TNM阶段的乳腺癌。接受者操作特征曲线分析循环的能力mir - 188 - 5 - p区分乳腺癌患者和非癌变患者或患者乳房纤维腺瘤产生相应的区域曲线下的0.894和8.814。高度恶性肿瘤中表达下调吗行mda - mb - 231相对于恶性越少MCF-7细胞。通过使转染细胞与模仿抑制剂发现mir - 188 - 5 - p抑制乳腺癌癌症细胞增殖和迁移被其下游目标IL6ST介导。细胞内和exosomal比较mir - 188 - 5 - p水平表明,mir - 188 - 5 - p有选择地分为液来自mda - mb - 231细胞而不是来自MCF-7细胞。乳腺癌患者的血清减少健康对照组相比并没有差别相对于患者乳房纤维腺瘤。总结,mir - 188 - 5 - p在一个肿瘤抑制作用方式在乳腺癌进展和可能作为一种无创性早期诊断的生物标志物和在乳腺癌的治疗目标。

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