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首页> 外文期刊>Journal of cellular physiology. >Comparison of human-induced pluripotent stem cells and mesenchymal stem cell differentiation potential to insulin producing cells in 2D and 3D culture systems in vitro
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Comparison of human-induced pluripotent stem cells and mesenchymal stem cell differentiation potential to insulin producing cells in 2D and 3D culture systems in vitro

机译：人类多能干细胞的比较和间充质干细胞分化可能在2 d和3 d胰岛素生产细胞体外培养系统

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摘要

Diabetes is one of the most common diseases in the world that is chronic, progressive, and costly, and causes many complications. Common drug therapies are not able to cure it, and pancreas transplantation is not responsive to the high number of patients. The production of the insulin producing cells (IPCs) from the stem cells in the laboratory and their transplantation to the patient's body is one of the most promising new approaches. In this study, the differentiation potential of the induced pluripotent stem cells (iPSCs) and mesenchymal stem cells (MSCs) into IPCs was compared to each other while cultured on poly(lactic-co-glycolic) acid (PLGA)/ polyethylene glycol (PEG) nanofibrous scaffold as a 3D substrate and tissue culture polystyrene (TCPS) as a 2D substrate. Although the expression level of the insulin, Glut2 and pdx-1 genes in stem cells cultured on 3D substrate was significantly higher than the stem cells cultured on 2D substrate, the highest expression level of these genes was detected in the iPSCs cultured on PLGA-PEG. Insulin and C-peptide secretions from differentiated cells were also investigated and the results showed that secretions in cultured iPSCs on the PLGA-PEG were significantly higher than cultured iPSCs on the TCPS and cultured MSCs on both PLGA-PEG and TCPS. In addition, insulin protein was also expressed in the cultured iPSCs on the PLGA-PEG significantly higher than cultured MSCs on the PLGA-PEG. It can be concluded that differentiation potential of iPSCs into IPCs is significantly higher than human MSCs at both 2D and 3D culture systems.
机译：糖尿病是最常见的疾病之一世界长期的、进步的和昂贵的,并导致许多并发症。治疗无法治愈它,胰腺移植是驴唇不对马嘴高患者的数量。产生细胞(ipc)的干细胞实验室和移植病人的身体是最有前途的新之一方法。诱导多能干细胞的潜力(万能)和间充质干细胞(msc)ipc与对方在培养保利(lactic-co-glycolic)酸(PLGA) /聚乙二醇(PEG) nanofibrous脚手架一个3 d聚苯乙烯基质和组织文化(安全和)2 d衬底。水平的胰岛素,Glut2 pdx-1基因干细胞培养的3 d衬底明显高于干细胞培养在2 d衬底,表达水平最高的发现这些基因在培养的细胞则PLGA-PEG。分化细胞也被调查结果表明,分泌物培养万能的PLGA-PEG明显更高比培养细胞则在安全和和培养的mscPLGA-PEG和安全和。蛋白质是培养的细胞则也表示PLGA-PEG明显高于培养PLGA-PEG msc。得出的结论是,万能干细胞的分化潜力到ipc明显高于人类的msc在2 d和3 d的文化系统。

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