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首页> 外文期刊>Applied biochemistry and biotechnology, Part A. enzyme engineering and biotechnology >Horticultural Waste as the Substrate for Cellulase and Hemicellulase Production by Trichoderma reesei Under Solid-State Fermentation
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Horticultural Waste as the Substrate for Cellulase and Hemicellulase Production by Trichoderma reesei Under Solid-State Fermentation

机译：园艺废弃物作为里氏木霉固态发酵生产纤维素酶和半纤维素酶的底物

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摘要

Horticultural waste in wood chips form collected from a landscape company in Singapore was utilized as the substrate for the production of cellulase and hemicellulase under solid-state fermentation by Trichoderma reesei RUT-C30. The effects of substrate pretreatment methods, substrate particle size, incubation temperature and time, initial medium pH value, and moisture content on cellulase and hemicellulase production were investigated. Enzyme complex was obtained at the optimal conditions. This enzyme mixture contained FPase (15.0 U/g substrate dry matter, SDM), CMCase (90.5 U/g SDM), β-glucosidase (61.6 U/g SDM), xylanase (52.1 U/g SDM), and β-xylosidase (10.4 U/g SDM). The soluble protein concentration in the enzyme complex was 26.1 mg/g SDM. The potential of the crude enzyme complex produced was demonstrated by the hydrolysis of wood chips, wood dust, palm oil fiber, and waste newspaper. The performance of the crude enzyme complex was better than the commercial enzyme blend.
机译：从里氏木霉RUT-C30固态发酵下，从新加坡一家园林公司收集的木片形式的园艺废物被用作生产纤维素酶和半纤维素酶的基质。研究了底物预处理方法，底物粒径，孵育温度和时间，初始培养基pH值以及水分含量对纤维素酶和半纤维素酶生产的影响。在最佳条件下获得了酶复合物。该酶混合物包含FPase（15.0 U / g底物干物质SDM），CMCase（90.5 U / g SDM），β-葡萄糖苷酶（61.6 U / g SDM），木聚糖酶（52.1 U / g SDM）和β-木糖苷酶（10.4 U / g SDM）。酶复合物中的可溶性蛋白质浓度为26.1 mg / g SDM。木片，木屑，棕榈油纤维和废报纸的水解证明了所产生的粗酶复合物的潜力。粗酶复合物的性能优于市售酶混合物。

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