...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Genetic testing and molecular biomarkers >Multiplex allele-specific amplification from whole blood for detecting multiple polymorphisms simultaneously
【24h】

Multiplex allele-specific amplification from whole blood for detecting multiple polymorphisms simultaneously

机译：从整个多元allele-specific放大血液检测多个多态性同时

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Allele-specific amplification on the basis of polymerase chain reaction (PCR) has been widely used for single-nucleotide polymorphism (SNP) genotyping. However, the extraction of PCR-compatible genomic DNA from whole blood is usually required. This process is complicated and tedious, and is prone to cause cross-contamination between samples. To facilitate direct PCR amplification from whole blood without the extraction of genomic DNA, we optimized the pH value of PCR solution and the concentrations of magnesium ions and facilitator glycerol. Then, we developed multiplex allele-specific amplifications from whole blood and applied them to a case-control study. In this study, we successfully established triplex, five-plex, and eight-plex allele-specific amplifications from whole blood for determining the distribution of genotypes and alleles of 14 polymorphisms in 97 gastric cancer patients and 141 healthy controls. Statistical analysis results showed significant association of SNPs rs9344, rs1799931, and rs1800629 with the risk of gastric cancer. This method is accurate, time-saving, cost-effective, and easy-to-do, especially suitable for clinical prediction of disease susceptibility.
机译：Allele-specific放大的基础上聚合酶链反应(PCR)已被广泛用于单核苷酸多态性(SNP)基因分型。PCR-compatible从全血基因组DNA通常是必需的。乏味,容易引起样品之间的交叉污染。便于直接从整个PCR扩增血没有基因组DNA的提取,我们优化的pH值和PCR的解决方案镁离子浓度和促进者甘油。从全血allele-specific放大和应用病例对照研究。研究中,我们成功地建立了三层,five-plex, eight-plex allele-specific为确定放大从全血基因型和等位基因的分布14多态性胃癌患者和97年141健康对照组。结果显示重要的协会的snprs9344、rs1799931 rs1800629的风险胃癌。节省时间,节省成本,而且容易做到,特别适用于临床的预测疾病的易感性。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号