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首页> 外文期刊>Genetic testing and molecular biomarkers >The Association of MMP-9 Enzyme Activity, MMP-9 C1562T Polymorphism, and MMP-2 and -9 and TIMP-1, -2, -3, and -4 Gene Expression in Lung Cancer
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The Association of MMP-9 Enzyme Activity, MMP-9 C1562T Polymorphism, and MMP-2 and -9 and TIMP-1, -2, -3, and -4 Gene Expression in Lung Cancer

机译：MMP-9 MMP-9协会酶活性C1562T多态性,MMP-2 9 TIMP-1,2、3和4在肺癌基因表达

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摘要

Aim: To investigate the association of gene expression of MMP-2 and -9, and TIMP-1, -2, -3, and -4 and polymorphism frequencies of MMP-9 C1562T and plasma MMP-9 enzyme activity in lung cancer patients. Methods: In this study, DNA and RNA samples were extracted from peripheral blood of 300 subjects (200 lung cancer patients and 100 controls). MMP-9 C1562T polymorphism was determined using restriction fragment length polymorphism (RFLP) method, and expression of MMP-2 and -9, TIMP-1, -2, -3, and -4 was determined using reverse transcriptase polymerase chain reaction. Plasma MMP-9 enzyme activity levels were measured using enzymelinked immunosorbent assay. Results and Conclusion: The frequencies of C1562T genotypes were found to be CC 67%, CT 30%, and TT 3% in the control group and CC 75%, CT 24%, and TT 1% in the patient group. It was determined that CC genotype frequency increases significantly in patients according to control group. Plasma MMP-9 enzyme activity levels increased in patients with lung cancer compared to the control group. The cut-off value of MMP-9 enzyme activity was determined as 7.76 ng/mL by receiver operating characteristics curve analysis. The sensitivity, specificity, positive predictive value, and negative predictive value were 77%, 51%, 75.9%, and 52.6%, respectively. The expression of MMP-2 and TIMP-1 was found to be higher in lung cancer patients. Finally, we claim that determination of MMP-9 enzyme levels and expression of MMP-2 and -9 and TIMP-1 can be used as a marker in lung cancer.
机译：目的:探讨基因的协会MMP-2和9的表达,TIMP-1, 2, 3,和4和MMP-9多态性频率C1562T和等离子MMP-9酶活性在肺癌症患者。RNA从外周血中提取的样本300例(200个肺癌病人和100个控制)。使用限制片段长度决定的多态性(RFLP)方法和表达TIMP-1 MMP-2, 9日,2,3,4决定使用逆转录酶聚合酶连锁反应。使用enzymelinked水平测定免疫吸附试验。发现了C1562T基因型的频率CC 67%, CT 30%, TT对照组的3%TT和CC 75%, CT 24%,病人的1%组。频率显著增加患者根据对照组。活动水平增加患者的肺癌症与对照组相比。价值MMP-9酶活动的决心7.76 ng / mL的接收机工作特性曲线分析。阳性预测值和消极预测值分别为77%,51%,75.9%,52.6%,分别。在肺癌患者被发现更高。最后,我们认为MMP-9的决心酶水平和MMP-2和9的表达TIMP-1可以作为肺癌的一个标志。

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