PCR-Quality DNA Isolation from Human Bronchial Aspirates and Buccal and Eyelid Swabs by a Simple Procedure Based on Alkaline Lysis

Bozica Vrabec Branica; Dinko Mitrecic; Sandra GrgicSilvana Smojver JezekNabil ChalfeSrecko Gajovie

首页> 外文期刊>Genetic testing and molecular biomarkers >PCR-Quality DNA Isolation from Human Bronchial Aspirates and Buccal and Eyelid Swabs by a Simple Procedure Based on Alkaline Lysis

【24h】

PCR-Quality DNA Isolation from Human Bronchial Aspirates and Buccal and Eyelid Swabs by a Simple Procedure Based on Alkaline Lysis

机译：人类支气管PCR-Quality DNA隔绝一个简单的吸入物、颊和眼睑拭子基于碱性溶解的过程

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

There are only a few systematic reports about DNA extraction from routine diagnostic cytological specimens. An inevitable drawback of such techniques is increased spending of time and funds required for obligatory DNA purification. To implement a simple protocol for human DNA isolation from cytological specimens related to lung cancer, bronchial aspirates together with samples collected by swabbing of the inner cheek and eyelid were used. By combining alkaline and temperature lyses it was possible to isolate DNA solution ready for PCR in less than an hour. Testing the method used for amplification of sex chromatin gene fragments showed that it is highly efficient. The presented protocol preserves high-quality DNA that is suitable for PCR-based assays.

机译：只有少数系统关于DNA的报告提取从日常诊断细胞学标本。技术的时间和增加支出基金所需的DNA纯化。实现一个简单的人类DNA的协议从细胞学标本与隔离肺癌、支气管一起吸入等收集的样本内的抽汲的脸颊和眼睑。温度溶解分离是可能的DNA解决方案准备PCR在不到一个小时。测试的方法用于放大性染色质基因片段显示,它是高度非常高效。高质量的DNA,适用于pcr化验。

著录项

来源
《Genetic testing and molecular biomarkers》 |2009年第6期|799-802|共4页
作者
Bozica Vrabec Branica; Dinko Mitrecic; Sandra GrgicSilvana Smojver JezekNabil ChalfeSrecko Gajovie;
展开▼
作者单位

Department of Pulmology, University Hospital for Lung Diseases "Jordanovac," Zagreb, Croatia;

Croatian Institute for Brain Research, School of Medicine University of Zagreb, Zagreb, Croatia;

Department of Cytology, University Hospital for Lung Diseases "Jordanovac," Zagreb, Croatia;

展开▼
收录信息
原文格式 PDF
正文语种英语
中图分类
关键词
Buccal Surface; Chromatin; Aspiration procedureEyelidsFundsTemperatureAlkalineSex ChromatinDNA isolationSwabTissue LysisDNA extraction;

机译：颊面、染色质、抱负procedureEyelidsFundsTemperatureAlkalineSex提取;

PCR-Quality DNA Isolation from Human Bronchial Aspirates and Buccal and Eyelid Swabs by a Simple Procedure Based on Alkaline Lysis

摘要

著录项

相关主题

期刊订阅