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首页> 外文期刊>Aquatic Toxicology >Determination of photosynthetic and enzymatic biomarkers sensitivity used to evaluate toxic effects of copper and fludioxonil in alga Scenedesmus obliquus
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Determination of photosynthetic and enzymatic biomarkers sensitivity used to evaluate toxic effects of copper and fludioxonil in alga Scenedesmus obliquus

机译：测定光合作用和酶促生物标志物的敏感性，以评估铜和氟迪松对藻类斜生藻的毒性作用

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摘要

Modulated RAM fluorometry and Plant Efficiency Analyser methods were used to investigate photosynthetic fluorescence parameters of alga Scenedesmus obliquus exposed to inhibitory effect of fungicides copper sulphate and fludioxonil (N-(4-nitrophenyl)-N'-propyl-uree), The change of those parameters were studied when alga S. obliquus have been exposed during 48 h to different concentrations of fungicides (1, 2 and 3 mg l~(-1)). Under the same condition, enzymatic activities of catalase, ascorbate peroxidase, glutathione reductase and glutathione S-transferase were investigated to evaluate antioxidative response to fungicides effects. The change of sensitivity of those parameters was dependent to the mode of fungicide action, their concentration and time of exposure. Foi copper effects, the most indicative photosynthetic biomarkers were parameters Q_N as non-photochemical fluorescence quenching, Q_(Emax) as the proton induced fluorescence quenching and ABS/RC as the antenna size per photosystem II reaction center Copper induced oxidative stress was indicated by increased activity of catalase serving as the most sensitive and valuable enzymatic biomarker On the other hand, fludioxonil effect on photosynthetic parameters was very negligible and consequently not very useful as biomarkers. However, fludioxonil induced strong antioxidative activities associated with cytosol enzymes, as we found for catalase, ascorbate peroxidase and glutathione S-transferase activities. By obtained results, we may suggest for the activation of those enzymes to be sensitive and valuable biomarkers of oxidative stress induced by fludioxonil. Determination of biomarkers sensitivity may offer advantages in providing real criteria to use them for ecotoxicological diagnosticstudies.
机译：使用调制的RAM荧光法和植物效率分析仪方法研究了斜生藻藻的光合荧光参数，该藻暴露于杀菌剂硫酸铜和氟迪松（N-（4-硝基苯基）-N'-丙基脲）的抑制作用下，其变化研究了斜生海藻在48小时内暴露于不同浓度的杀菌剂（1、2和3 mg l〜（-1））时的参数。在相同条件下，研究过氧化氢酶，抗坏血酸过氧化物酶，谷胱甘肽还原酶和谷胱甘肽S-转移酶的酶活性，以评估对杀菌剂作用的抗氧化反应。这些参数的敏感性变化取决于杀菌剂作用的方式，其浓度和暴露时间。 Foi铜效应，最具指示性的光合生物标记是每个光系统II反应中心的参数Q_N为非光化学荧光猝灭，Q_（Emax）为质子诱导的荧光猝灭，而ABS / RC为天线尺寸II过氧化氢酶的活性作为最敏感和最有价值的酶生物标记物。另一方面，氟地莫尼对光合作用参数的影响可忽略不计，因此作为生物标记物用途不大。然而，如我们发现过氧化氢酶，抗坏血酸过氧化物酶和谷胱甘肽S-转移酶活性，氟地西尼可诱导与胞浆酶相关的强抗氧化活性。通过获得的结果，我们可能建议这些酶的激活是氟迪莫尼诱导的氧化应激的敏感和有价值的生物标记。确定生物标志物的敏感性可能在提供将其用于生态毒理学诊断研究的真实标准方面具有优势。

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