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首页> 外文期刊>Aquatic Toxicology >Cellular biomarkers of exposure and biological effect in hepatocytes of turbot (Scophthalmus maximus) exposed to Cd, Cu and Zn and after depuration
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Cellular biomarkers of exposure and biological effect in hepatocytes of turbot (Scophthalmus maximus) exposed to Cd, Cu and Zn and after depuration

机译：净化，净化和净化后镉，铜和锌对菱turbo（Scophthalmus maximus）肝细胞的暴露及生物学效应的细胞生物标志物

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Cellular biomarkers of exposure and biological effects were measured in hepatocytes of turbot exposed to either Cd, Cu or Zn at concentrations of 1 and 10 mg/1 seawater for 7 days and after depuration for 14 days Metal content in hepatocyte lysosomeswas determined by image analysis after autometallography (AMG) as volume density of autometallographed black silver deposits (Vv_(BSD). Metallothionein (MT) levels were quantified on liver sections by microdensitometry after immunohistochemical stainingwith a polyclonal anti cod-MT antibody (MT-OD), and in the cytosolic fraction of hepatocytes by difference pulse polarography (MT-DPP). Lysosomal structural changes (lysosomal volume, surface and numerical densities—Vv_(LYS), Sv_(LYS) and Nv_(LYS), andsurface-to-volume ratio S/V_(Lys)) were quantified by image analysis after demonstration of p-glucuronidase activity on liver cryotome sections Vacuolisation produced by metal-exposure in hepatocytes was quantified by stereology as volume density of vacuoles (VVVAC) Exposure time and metal concentrations significantly affected VVBSD in lysosomes, MT levels and the degree of vacuolisation after 1 h and 1 day exposure to the three metals. The highest VVBSD, MT and VV_(VAC) values were recorded after 7 days exposure in all cases, MT-OD and MT-DPP were significantly correlated with VVBSD Vv_(LYS) in hepatocytes increased significantly after exposure to the metals. Exposure biomarkers returned to control values after depuration with the exception of those turbots that had been exposed to 10 mg Cd/1. Alike, Vviys and Sv_(lys) (Cu exposure) and NV_(LYS) (Cd and Zn exposures) returned to control values after depuration. It has been therefore demonstrated that the biomarkers used are reversible and return towards control levels once metal exposure ceases. Overall, it is concluded that VVBSD, MT-levels and lysosomal responses are valuable biomarkers to assess metal exposure and its effects in turbot, although in quantitative terms the biomarker response variedbetween metals.
机译：在暴露于Cd，Cu或Zn浓度分别为1和10 mg / 1海水7天和净化14天后的菱形肝细胞中，测量其暴露和生物学效应的细胞生物标志物，通过图像分析确定肝细胞溶酶体中的金属含量。自体金相学（AMG）作为自体金相学黑银沉积物（Vv_（BSD））的体积密度。用多克隆抗cod-MT抗体（MT-OD）免疫组化染色后，通过显微光密度法在肝脏切片上定量金属硫蛋白（MT）的水平，并在细胞溶质中通过差动脉冲极谱法（MT-DPP）检测肝细胞的分数。溶酶体的结构变化（溶酶体的体积，表面和数值密度-Vv_（LYS），Sv_（LYS）和Nv_（LYS）以及表面体积比S / V_（ Lys））在显示了肝脏冷冻切片机切片上的p-葡萄糖醛酸酶活性后，通过图像分析进行了定量分析，通过立体定量定量了肝细胞中金属暴露所产生的真空化，作为体积密度液泡y（VVVAC）暴露时间和金属浓度显着影响溶酶体中的VVBSD，MT水平以及暴露于三种金属1 h和1天后的液化程度。在所有情况下暴露7天后，记录到最高的VVBSD，MT和VV_（VAC）值，暴露于金属后，肝细胞中MT-OD和MT-DPP与VVBSD Vv_（LYS）显着相关。净化后，暴露的生物标志物恢复到控制值，但已暴露于10 mg Cd / 1的大菱turbo除外。同样，净化后，Vviys和Sv_（lys）（铜暴露）和NV_（LYS）（镉和锌暴露）恢复到控制值。因此，已经证明所使用的生物标志物是可逆的，并且一旦金属暴露停止就恢复到对照水平。总的来说，可以得出结论，VVBSD，MT水平和溶酶体反应是评估金属暴​​露及其在大菱t中的作用的有价值的生物标志物，尽管在数量上金属间的生物标志物反应有所不同。

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