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首页> 外文期刊>Aquatic Toxicology >Zebrafish embryos sequester and retain petrochemical combustion products: developmental and transcriptome consequences.
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Zebrafish embryos sequester and retain petrochemical combustion products: developmental and transcriptome consequences.

机译：斑马鱼胚胎螯合并保留石化燃烧产物：发育和转录组的后果。

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摘要

Zebrafish embryos are a model for studying effects of environmental stressors on development. Incomplete combustion of the environmentally relevant volatile petrochemical, 1,3-butadiene (BD) yields butadiene soot (BDS) nanoparticles, to which polynuclear aromatic hydrocarbons (PAHs) are adsorbed. In mammalian cells these PAHs are concentrated in lipid droplets and trigger up-regulation of biotransformation, oxidative stress and inflammatory genes. The present study was designed to determine whether: (a) PAH-rich BDS elicits alterations in zebrafish embryo development; (b) BDS-exposed zebrafish embryos sequester PAHs in select tissues; and (c) developmental abnormalities are correlated with altered gene expression patterns. 1-day old zebrafish embryos were exposed for 48 h to BDS (0, 6, 30 or 60 micro g/ml) sprinkled on the water surface. PAH localization was tracked by fluorescence. Developmental responses (pericardial edema, yolk sac swelling, axial malformations) were monitored by microscopy. Gene expression changes were assessed by gene microarray and qRT-PCR. Our results show that PAHs localized with endogenous lipids in the yolk sac and in hatching gland cells. PAHs were retained at least 8 days after exposures ended. Dose-dependent pericardial and yolk sac edema and axial malformations were prominent and accompanied by up-regulation of biotransformation and oxidative stress gene cascades. Thus, zebrafish embryos should be useful for predicting the potential for developmental toxicity following exposure to PAH-rich petrochemical soots, e.g., those arising from attempts at oil spill remediation by combustion.
机译：斑马鱼胚胎是研究环境应激源对发育影响的模型。与环境有关的挥发性石化产品1,3-丁二烯（BD）的不完全燃烧会产生丁二烯烟灰（BDS）纳米颗粒，多核芳香烃（PAHs）被吸附到该纳米颗粒上。在哺乳动物细胞中，这些PAHs集中在脂质液滴中，并触发生物转化，氧化应激和炎症基因的上调。本研究旨在确定是否：（a）富含PAH的BDS引起斑马鱼胚胎发育的改变; （b）暴露于BDS的斑马鱼胚胎在特定组织中螯合PAH; （c）发育异常与基因表达模式改变有关。将1天大的斑马鱼胚胎暴露于撒在水面上的BDS（0、6、30或60 micro g / ml）48小时。通过荧光追踪PAH的定位。通过显微镜监测发育反应（心包水肿，卵黄囊肿胀，轴向畸形）。通过基因芯片和qRT-PCR评估基因表达变化。我们的结果表明，PAHs定位于卵黄囊和孵化腺细胞中的内源性脂质。暴露结束后至少保留8天PAHs。剂量依赖性心包和卵黄囊水肿和轴向畸形突出，并伴有生物转化和氧化应激基因级联的上调。因此，斑马鱼胚胎应可用于预测在暴露于富含PAH的石油化学烟灰（例如，试图通过燃烧补救溢油而产生的烟灰）后产生发育毒性的可能性。

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