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首页> 外文期刊>Acta crystallographica. Section D, Structural biology >Radiation-induced defects in protein crystals observed by X-ray topography
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Radiation-induced defects in protein crystals observed by X-ray topography

机译：辐射诱导蛋白质晶体缺陷观察到x射线地形

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摘要

The characterization of crystal defects induced by irradiation, such as X-rays, charged particles and neutrons, is important for understanding radiation damage and the associated generation of defects. Radiation damage to protein crystals has been measured using various methods. Until now, these methods have focused on decreased diffraction intensity, volume expansion of unit cells and specific damage to side chains. Here, the direct observation of specific crystal defects, such as dislocations, induced by X-ray irradiation of protein crystals at room temperature is reported. Dislocations are induced even by low absorbed doses of X-ray irradiation. This study revealed that for the same total absorbed dose, the formation of defects appears to critically depend on the dose rate. The relationship between dislocation energy and dose energy was analyzed based on dislocation theory associated with elasticity theory for crystalline materials. This demonstration of the crystal defects induced by X-ray irradiation could help to understand the underlying mechanisms of X-ray-induced radiation damage.
机译：引起的晶体缺陷的表征辐照,如x射线,带电粒子和中子,对理解很重要辐射损伤和相关的一代缺陷。使用各种方法测量。这些方法都集中在减少衍射强度,体积膨胀的单位细胞和具体损伤侧链。直接观察特定的晶体缺陷,x射线引起的混乱等辐照蛋白质晶体的房间温度是报道。即使是低吸收剂量的x射线辐射。这项研究显示,同样的总数吸收剂量,就会出现缺陷的形成极度依赖于剂量率。位错的能量和剂量之间的关系基于位错理论能量进行了分析与弹性理论结晶材料。缺陷引起的x射线辐照能有所帮助理解底层机制X-ray-induced辐射损伤。

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