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首页> 外文期刊>Aquatic Biology >Isolation of seaweed-associated bacteria and their morphogenesis-inducing capability in axenic cultures of the green alga Ulva fasciata
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Isolation of seaweed-associated bacteria and their morphogenesis-inducing capability in axenic cultures of the green alga Ulva fasciata

机译：藻类Ulva fasciata的无性培养中海藻相关细菌的分离及其诱导形态发生的能力

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摘要

In the marine environment, the bacteria that colonize the surface of a seaweed affect its growth and development. In the laboratory, several seaweeds do not develop normal foliose thalli in axenic cultures, but adopt an atypical morphology. Adding certain bacteria to the culture, however, can result in reversion to the normal morphology. The aim of the present study was to examine the effects of various algal-associated bacteria on the growth, morphology, and reproduction of the green alga Ulva fasciata. In axenic culture without added bacteria, U. fasciata grew into undifferentiated tissue initially and later developed into a tubular thallus. Of the 53 bacterial isolates we obtained from different species of Ulva and Gracilaria, only 5 were capable of inducing differentiation and subsequent growth in U. fasciata cultured in axenic conditions. One bacterial isolate (which we later identified as Marinomonas sp.) was more effective than the others in inducing morphogenesis and growth in U. fasciata. The physical association of bacterial cells with thalli was found to be crucial for the induction of U. fasciata foliose morphology, growth, and spore release. The culture filtrates from the 5 individual bacterial isolates and from a consortium of all 5 of them had less effect on morphogenesis and growth of U. fasciata than the bacteria themselves had, but more effect than the control (no bacteria added). Analysis of partial 16S rRNA gene sequences from all 5 isolates with morphogenesis-inducing ability led us to identify them as Marinomonas sp. and Bacillus spp. We found that the physical association of bacteria is essential for normal growth of the seaweed, suggesting a symbiotic interaction between seaweed and bacteria.
机译：在海洋环境中，定居在海藻表面的细菌会影响其生长和发育。在实验室中，几种海藻在无菌培养中不会发育出正常的叶状藻，而是呈非典型形态。但是，向培养物中添加某些细菌可能会导致恢复到正常形态。本研究的目的是检查各种藻类相关细菌对绿藻Ulva fasciata的生长，形态和繁殖的影响。在不添加细菌的无菌培养中，筋膜螺旋藻最初生长为未分化的组织，随后发育为管状th。我们从不同种类的Ulva和Gracilaria获得的53种细菌分离物中，只有5种能够诱导在未成熟条件下培养的U. fasciata的分化和随后的生长。一种细菌分离物（我们后来鉴定为Marinomonas sp。）比其他细菌分离物更能诱导筋膜藻的形态发生和生长。发现细菌细胞与thalli的物理联系对于诱导U. fasciata叶形，生长和孢子释放至关重要。来自5个单独的细菌分离物以及来自所有5个细菌的财团的培养物滤液对筋膜螺旋藻的形态发生和生长的影响比细菌本身对细菌的影响要小，但对对照的影响更大（不添加细菌）。对来自所有5个具有形态发生诱导能力的菌株的16S rRNA基因部分序列的分析使我们将其鉴定为Marinomonas sp。和芽孢杆菌属。我们发现细菌的物理缔合对于海藻的正常生长至关重要，表明海藻与细菌之间存在共生相互作用。

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