Crystal structure of the domain-swapped dimeric maltodextrin-binding protein MalE from Salmonella enterica

Wang Lulu; Bu Tingting; Bai XueHe ShanruZhang JieJin LimingLiu BaoquanDong YueshengHa Nam ChulQuan ChunshanNam Ki HyunXu Yongbin

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Crystal structure of the domain-swapped dimeric maltodextrin-binding protein MalE from Salmonella enterica

机译：晶体结构的domain-swapped二聚的maltodextrin-binding沙门氏菌蛋白质男子enterica

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MalE is a maltose/maltodextrin-binding protein (MBP) that plays a critical role in most bacterial maltose/maltodextrin-transport systems. Previously reported wild-type MBPs are monomers comprising an N-terminal domain (NTD) and a C-terminal domain (CTD), and maltose-like molecules are recognized between the NTD and CTD and transported to the cell system. Because MBP does not undergo artificial dimerization, it is widely used as a tag for protein expression and purification. Here, the crystal structure of a domain-swapped dimeric MalE from Salmonella enterica (named SeMalE) in complex with maltopentaose is reported for the first time, and its structure is distinct from typical monomeric MalE family members. In the domain-swapped dimer, SeMalE comprises two subdomains: the NTD and CTD. The NTD and CTD of one molecule of SeMalE interact with the CTD and NTD of the partner molecule, respectively. The domain-swapped dimeric conformation was stabilized by interactions between the NTDs, CTDs and linkers from two SeMalE molecules. Additionally, a maltopentaose molecule was found to be located at the interface between the NTD and CTD of different SeMalE molecules. These results provide new insights that will improve the understanding of maltodextrin-binding MalE proteins.

机译：男性是麦芽糖/ maltodextrin-binding蛋白质(MBP)发挥了至关重要的作用细菌麦芽糖/ maltodextrin-transport系统。之前报道野生型单体MBPs组成的n端结构域(元)和一个c端域(CTD), maltose-like被忽视的热带病和CTD之间的分子识别和运输到细胞系统。不接受人工二聚作用,它是什么广泛用作标记蛋白表达净化。沙门氏菌domain-swapped二聚的男子血清(名为SeMalE)在复杂maltopentaose首次报道它的结构是不同于典型的单节的男性家庭成员。SeMalE包含两个子域:被忽视的热带病和CTD。的被忽视的热带病和CTD SeMalE分子之一与连续油管和被忽视的热带病的伙伴分子,分别。二聚的构象稳定了之间的相互作用被忽略,连续油管和连接器从两个SeMalE分子。maltopentaose分子被发现位于的被忽视的热带病和连续油管之间的接口不同SeMalE分子。将提高理解的新见解maltodextrin-binding男性蛋白质。

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来源
《Acta crystallographica. Section D, Structural biology》 |2022年第5期|613-622|共10页
作者
Wang Lulu; Bu Tingting; Bai XueHe ShanruZhang JieJin LimingLiu BaoquanDong YueshengHa Nam ChulQuan ChunshanNam Ki HyunXu Yongbin;
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作者单位

Coll Life Sci,Dalian Minzu Univ;

Sch Life Sci & Biotechnol,Dalian Univ Technol;

Coll Agr & Life Sci,Seoul Natl UnivDept Life Sci,Pohang Univ Sci & Technol;

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正文语种英语
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关键词
maltopentaose; neutron transmutation doping; Salmonella entericaMaltoseconductivity temperature depthCharge transfer devicesmalecrystal structure;

机译：maltopentaose;中子嬗变沙门氏菌entericaMaltoseconductivity掺杂;温度depthCharge转移devicesmalecrystal结构;

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Crystal structure of the domain-swapped dimeric maltodextrin-binding protein MalE from Salmonella enterica

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