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首页> 外文期刊>Acta crystallographica. Section D, Structural biology >Structural studies of a novel auxiliary-domain-containing phenylalanine hydroxylase from Bacillus cereus ATCC 14579
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Structural studies of a novel auxiliary-domain-containing phenylalanine hydroxylase from Bacillus cereus ATCC 14579

机译：一种新型结构的研究auxiliary-domain-containing苯丙氨酸写明ATCC 14579羟化酶从蜡样芽胞杆菌

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摘要

Phenylalanine hydroxylase (PAH), which belongs to the aromatic amino-acid hydroxylase family, is involved in protein synthesis and pyomelanine production through the hydroxylation of phenylalanine to tyrosine. In this study, the crystal structure of PAH from Bacillus cereus ATCC 14579 (BcPAH) with an additional 280 amino acids in the C-terminal region was determined. The structure of BcPAH consists of three distinct domains: a core domain with two additional inserted alpha-helices and two novel auxiliary domains: BcPAH-AD1 and BcPAH-AD2. Structural homologues of BcPAH-AD1 and BcPAH-AD2 are known to be involved in mRNA regulation and protein-protein interactions, and thus it was speculated that BcPAH might utilize the auxiliary domains for interaction with its partner proteins. Furthermore, phylogenetic tree analysis revealed that the three-domain PAHs, including BcPAH, are completely distinctive from both conventional prokaryotic PAHs and eukaryotic PAHs. Finally, biochemical studies of BcPAH showed that BcPAH-AD1 might be important for the structural integrity of the enzyme and that BcPAH-AD2 is related to enzyme stability and/or activity. Investigations into the intracellular functions of the two auxiliary domains and the relationship between these functions and the activity of PAH are required.
机译：苯丙氨酸羟化酶(PAH),这属于芳香族氨基酸羟化酶家族参与蛋白质合成和pyomelanine生产的羟基化苯丙氨酸,酪氨酸。从蜡样芽胞杆菌晶体结构的多环芳烃写明ATCC 14579 (BcPAH)和一个额外的280氨基酸酸c端地区的决心。BcPAH包括三个不同的结构域:与另外两个核心领域插入阿尔法螺旋和两个新颖的辅助域:BcPAH-AD1 BcPAH-AD2。同系物BcPAH-AD1和BcPAH-AD2是已知的参与mRNA和监管蛋白质的相互作用,因此推测BcPAH可能利用辅助域名与合作伙伴的互动蛋白质。显示了物种三域分类多环芳烃,其中包括从两个BcPAH,完全是独特的传统的原核的多环芳烃和真核生物多环芳烃。表明BcPAH-AD1可能是重要的酶的结构完整性BcPAH-AD2相关酶和/或稳定活动。两个辅助域和功能这些功能和之间的关系活动的多环芳烃是必需的。

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