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首页> 外文期刊>Acta crystallographica. Section D, Structural biology >Single-support serial isomorphous replacement phasing
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Single-support serial isomorphous replacement phasing

机译：单一支架系列同形替换定相

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摘要

The use of single isomorphous replacement (SIR) has become less widespread due to difficulties in sample preparation and the identification of isomorphous native and derivative data sets. Non-isomorphism becomes even more problematic in serial experiments, because it adds natural inter-crystal non-isomorphism to heavy-atom-soaking-induced non-isomorphism. Here, a method that can successfully address these issues (and indeed can benefit from differences in heavy-atom occupancy) and additionally significantly simplifies the SIR experiment is presented. A single heavy-atom soak into a microcrystalline slurry is performed, followed by automated serial data collection of partial data sets. This produces a set of data collections with a gradient of heavy-atom occupancies, which are reflected in differential merging statistics. These differences can be exploited by an optimized genetic algorithm to segregate the pool of data sets into 'native' and 'derivative' groups, which can then be used to successfully determine phases experimentally by SIR.
机译：单一的使用同形替换(先生)已变得不那么普遍,由于困难吗样品制备和鉴定同晶型原生和衍生数据集。Non-isomorphism变得更成问题系列实验,因为它增加了自然晶间non-isomorphism来heavy-atom-soaking-induced non-isomorphism。一个方法,可以成功地解决这些问题(实际上可以从差异中受益在重原子占用),此外大大简化了实验是爵士提出了。微晶浆被执行时,紧随其后自动化的串行数据收集的部分数据集。梯度的重原子入住率,反映在微分合并统计。这些差异可以利用优化遗传算法分离池数据集的“本土”和“导数”组,然后可以用来成功确定实验阶段,先生。

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