...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta crystallographica. Section D, Structural biology >Structural variability of CG-rich DNA 18-mers accommodating double T-T mismatches
【24h】

Structural variability of CG-rich DNA 18-mers accommodating double T-T mismatches

机译：结构可变性18-mers CG-rich DNA适应双t t不匹配

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Solution and crystal data are reported for DNA 18-mers with sequences related to those of bacterial noncoding single-stranded DNA segments called repetitive extragenic palindromes (REPs). Solution CD and melting data showed that the CG-rich, near-palindromic REPs from various bacterial species exhibit dynamic temperature-dependent and concentration-dependent equilibria, including architectures compatible with not only hairpins, which are expected to be biologically relevant, but also antiparallel duplexes and bimolecular tetraplexes. Three 18-mer oligonucleotides named Hpar-18 (PDB entry 6rou), Chom-18 (PDB entry 6ros) and its brominated variant Chom-18Br (PDB entry 6ror) crystallized as isomorphic right-handed A-like duplexes. The low-resolution crystal structures were solved with the help of experimental phases for Chom-18Br. The center of the duplexes is formed by two successive T-T noncanonical base pairs (mismatches). They do not deform the double-helical geometry. The presence of T-T mismatches prompted an analysis of the geometries of these and other noncanonical pairs in other DNA crystals in terms of their fit to the experimental electron densities (RSCC) and their geometric fit to the NtC (dinucleotide conformational) classes (https://dnatco.datmos.org/). Throughout this work, knowledge of the NtC classes was used to refine and validate the crystal structures, and to analyze the mismatches.
机译：解决方案和DNA晶体数据报告18-mers与序列相关的细菌的单链DNA非编码片段被称为重复基因外回文(代表)。解决方案CD和数据表明,融化CG-rich near-palindromic代表从不同细菌物种表现出动态随温度而变的和浓度平衡,包括架构兼容不仅与发夹、预计将生物相关的,也是反平行的工器和双分子tetraplexes。18-mer寡核苷酸名为Hpar-18 (PDB条目6肾阳),Chom-18 (PDB项6 ros)和它的溴化变异Chom-18Br (PDB项6 ror)结晶是同构右手像工器。解决了在实验阶段的帮助吗Chom-18Br。由连续两个t t中的基础双(不匹配)。双螺旋几何学。不匹配引起的几何图形的分析这些和其他经典之中对其他DNA晶体的适合实验电子密度(RSCC)和他们的几何适合全国过渡委员会(二核苷酸构象)类(https://dnatco.datmos.org/)。“过渡委”类的工作,知识是用来优化和验证的晶体结构分析不匹配。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号