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首页> 外文期刊>Acta crystallographica. Section D, Structural biology. >Utilizing guanine-coordinated Zn~2+ ions todetermine DNA crystal structures bysingle-wavelength anomalous diffraction
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Utilizing guanine-coordinated Zn~2+ ions todetermine DNA crystal structures bysingle-wavelength anomalous diffraction

机译：利用guanine-coordinated锌~ 2 +离子确定DNA晶体结构bysingle-wavelength反常衍射

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摘要

The experimental phase determination of crystal structures of nucleic acids andnucleic acid-ligand complexes would benefit from a facile method. Even fordouble-stranded DNA, software-generated models are generally insufficientlyaccurate to serve as molecular replacement search models, necessitatingexperimental phasing. Here, it is demonstrated that Zn~2+ ions coordinated tothe N7 atom of guanine bases generate sufficient anomalous signal for single-wavelength anomalous diffraction (SAD) phasing of DNA crystal structures.Using zinc SAD, three crystal structures of double-stranded DNA oligomers,5'-AGGGATCCCT-3', 5'-GGGATCCC-3' and 5'-GAGGCCTC-3', were deter-mined. By determining the crystal structure of one of these oligomers,GAGGCCTC, in the presence of Mg~2+ instead of Zn~2+, it was demonstratedthat Zn~2+ is not structurally perturbing. These structures allowed the analysis ofstructural changes in the DNA on the binding of analogues of the naturalproduct mithramycin to two of these oligomers, AGGGATCCCT andGAGGCCTC. Zinc SAD may become a routine approach for determiningthe crystal structures of nucleic acids and their complexes with small molecules.
机译：晶体的实验阶段的决心核酸andnucleic结构acid-ligand复合物将受益于一个肤浅方法。软件生成的模型通常是insufficientlyaccurate作为分子更换搜索模型,necessitatingexperimental定相。表明,锌~ 2 +离子协调N7鸟嘌呤碱基产生足够的原子单波长反常的异常信号衍射(SAD)逐步的DNA晶体结构。双链DNA的结构5 ' -GAGGCCTC-3’,是开创性的。其中一个的晶体结构低聚物,GAGGCCTC毫克~ 2 +的存在而不是锌~ 2 +,这是demonstratedthat锌~ 2 +不是结构扰动。允许分析原理的变化DNA在绑定的类似物naturalproduct光神霉素两种低聚物,AGGGATCCCT andGAGGCCTC。成为determiningthe的常规方法核酸及其晶体结构与小分子复合物。

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