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首页> 外文期刊>Acta crystallographica. Section D, Structural biology. >Seeding from silica-reinforced lysozyme crystals for neutron crystallography
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Seeding from silica-reinforced lysozyme crystals for neutron crystallography

机译：播种从silica-reinforced溶菌酶晶体对中子晶体学

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摘要

The fragility of protein crystals plays an important role in the final quality of the diffraction data and therefore that of the derived three-dimensional structural model. The growth of protein crystals in gels of various natures has been shown to overcome this problem, facilitating the manipulation of the crystals; this is probably owing, amongst other factors, to the incorporation of the gel fibres within the body of the crystal. In this study, lysozyme crystals were grown in silica gel at a wide range of concentrations of up to 22%(v/v) to quantitatively determine the amount of gel incorporated into the crystal structure by means of thermogravimetric analysis. The interaction between the silica fibres and the lysozyme molecules within the crystals was also investigated using Raman spectroscopy and the direct influence on the crystalline protein stability was analysed using differential scanning calorimetry. Finally, the benefits of the use of gel-grown crystals to overgrow protein crystals intended for neutron diffraction are highlighted.
机译：蛋白质晶体的脆弱性具有重要作用的最终质量因此,衍射数据推导出三维结构模型。增长的蛋白质晶体不同的凝胶性质已经被证明可以克服这个问题,方便操作的晶体;这可能是由于,在其他因素内的凝胶纤维的掺入身体的晶体。晶体在广泛生长在硅胶的浓度高达22% (v / v)定量确定凝胶的数量通过合并到晶体结构热重分析。石英纤维和溶菌酶分子内的晶体也使用拉曼光谱和调查直接影响水晶的蛋白质使用微分稳定性进行了分析扫描量热法。使用gel-grown晶体生长过度的蛋白质晶体用于中子衍射突出显示。

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