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首页> 外文期刊>Acta crystallographica. Section D, Structural biology. >Towards a compact and precise sample holder for macromolecular crystallography
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Towards a compact and precise sample holder for macromolecular crystallography

机译：紧凑和精确的样品持有人大分子晶体学

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Most of the sample holders currently used in macromolecular crystallography offer limited storage density and poor initial crystal‐positioning precision upon mounting on a goniometer. This has now become a limiting factor at high‐throughput beamlines, where data collection can be performed in a matter of seconds. Furthermore, this lack of precision limits the potential benefits emerging from automated harvesting systems that could provide crystal‐position information which would further enhance alignment at beamlines. This situation provided the motivation for the development of a compact and precise sample holder with corresponding pucks, handling tools and robotic transfer protocols. The development process included four main phases: design, prototype manufacture, testing with a robotic sample changer and validation under real conditions on a beamline. Two sample‐holder designs are proposed: NewPin and miniSPINE. They share the same robot gripper and allow the storage of 36 sample holders in uni‐puck footprint‐style pucks, which represents 252 samples in a dry‐shipping dewar commonly used in the field. The pucks are identified with human‐ and machine‐readable codes, as well as with radio‐frequency identification (RFID) tags. NewPin offers a crystal‐repositioning precision of up to 10?μm but requires a specific goniometer socket. The storage density could reach 64 samples using a special puck designed for fully robotic handling. miniSPINE is less precise but uses a goniometer mount compatible with the current SPINE standard. miniSPINE is proposed for the first implementation of the new standard, since it is easier to integrate at beamlines. An upgraded version of the SPINE sample holder with a corresponding puck named SPINEplus is also proposed in order to offer a homogenous and interoperable system. The project involved several European synchrotrons and industrial companies in the fields of consumables and sample‐changer robotics. Manual handling of miniSPINE was tested at different institutes using evaluation kits, and pilot beamlines are being equipped with compatible robotics for large‐scale evaluation. A companion paper describes a new sample changer FlexED8 (Papp et al. , 2017, Acta Cryst. , D 73 , 841–851).
机译：目前大部分的样品持有人中使用大分子晶体学提供有限存储密度和可怜的初始晶体在安装在列车定位精度测角仪。在高吞吐量beamlines数据可以执行的集合秒。限制了新兴的潜在好处自动采集系统,可以提供水晶的位置信息,将进一步加强在beamlines对齐。的发展提供了动力紧凑和精确的样品架相应的妖精,处理工具和机器人转让协议。包括四个主要阶段:设计、原型与机械制造、测试样本改变和验证在实际条件下beamline。NewPin miniSPINE。钳子和允许存储的36个样本持有者进入大学非冰球足迹高风格妖精代表一个干航运杜瓦252个样本常用的领域。地理与人类和机器可读的地理编码,以及无线电频率识别(RFID)标签。水晶列车定位精度高达10吗?但是需要一个特定的测角仪插座。存储密度将达到64样品使用特殊的冰球为完全机器人处理而设计的。miniSPINE不太精确,但使用测角仪山与当前脊柱标准兼容。miniSPINE提出了第一个实施新的标准,因为它是在beamlines容易集成。版本的脊柱持有人与示例相应的冰球名叫SPINEplus也为了提供一个同质和求婚可互操作的系统。一些欧洲同步加速器和工业公司在消费品领域,样改变机器人。miniSPINE测试在不同的学院使用评估板,飞行员beamlines具备兼容的机器人大尺度评价。描述了一种新的样本改变FlexED8 (Papp等, 2017年,学报。

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