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首页> 外文期刊>Aquaculture >Development of a multiplex PCR to detect Kudoa spp. and to distinguish Kudoa septempunctata in olive flounder Paralichthys olivaceus
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Development of a multiplex PCR to detect Kudoa spp. and to distinguish Kudoa septempunctata in olive flounder Paralichthys olivaceus

机译：研发多重PCR以检测工藤属。并区分橄榄比目鱼Paralichthys olivaceus中的Kudoa septempunctata

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摘要

Since the identification of Kudoa septempunctata as a causative agent of human food poisoning, Kudoa infections have becomea serious concern in the aquaculture of olive flounder (Paralichthys olivaceus). In order to ensure the safety of cultured flounder, various methods have been developed to detect K. septempunctata. However, less attention has been paid for inspection and detection of other Kudoa species. To date, six Kudoa spp. have been reported from olive flounder, 3 species each fromthe trunkmuscle, 2 species fromthe brain, and one species from the heart. During our routine inspection of cultured olive flounder, we isolated several Kudoa species including Kudoa igami and Kudoa ogawai in addition to the list of previously reported species. In the present study, a multiplex PCR (mPCR) was developed to detect all Kudoa spp. known to date fromolive flounder in Japan. ThemPCR also differentiate K. septempunctata among other Kudoa spp. simultaneously. The developed mPCR successfully detected seven Kudoa spp. and differentiated K. septempunctata with a sensitivity of 103 myxospores. Thus, mPCR offers a reliable and relatively simple method to detect Kudoa infection in olive flounder, specifically aiming to identify K. septempunctata infection.
机译：自从鉴定出油菜苦菜是人类食物中毒的致病因素以来，油udo鱼的养殖已成为比目鱼（Paralichthys olivaceus）的严重关切。为了确保养殖比目鱼的安全性，已开发出多种方法来检测分离的K. septempunctata。但是，对其他Kudoa物种的检查和检测却很少受到关注。迄今为止，共有六个Kudoa物种。据报道，橄榄比目鱼有3种，躯干肌各有3种，大脑有2种，心脏有1种。在我们对养殖比目鱼的例行检查中，除先前报道的物种清单外，我们还分离了几种Kudoa物种，包括Kudoa igami和Kudoa ogawai。在本研究中，开发了一种多重PCR（mPCR）来检测所有的Kudoa spp。迄今在日本因臭比目鱼而闻名。该mPCR还区分了其他Kudoa spp中的K. septempunctata。同时。开发的mPCR成功检测到7个Kudoa spp。并以103个黏孢子的敏感性区分了K. septempunctata。因此，mPCR提供了一种可靠且相对简单的方法来检测橄榄比目鱼的库多氏菌感染，特别是旨在鉴定九球菌的感染。

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