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首页> 外文期刊>Journal of Cellular Physiology >P2Y12 shRNA treatment decreases SGC activation to relieve diabetic neuropathic pain in type 2 diabetes mellitus rats
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P2Y12 shRNA treatment decreases SGC activation to relieve diabetic neuropathic pain in type 2 diabetes mellitus rats

机译：P2Y12 shRNA治疗减少国网公司激活缓解2型糖尿病神经性疼痛糖尿病大鼠

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摘要

Diabetic neuropathic pain is a common complication of type 2 diabetes mellitus (DM). Activation of satellite glial cells (SGCs) in the dorsal root ganglia (DRG) plays a crucial role in neuropathic pain through the release of proinflammatory cytokines. The P2Y12 receptor is expressed in SGCs of the DRG. In this study, our aim was to investigate the role of the P2Y12 receptor on the pathological changes in diabetic neuropathic pain. The present study showed that diabetic neuropathic pain increased mechanical and thermal hyperalgesia in type 2?DM model rats. The results showed that the expression levels of P2Y12 messenger RNA (mRNA) and protein in DRG SGCs were increased in DM model rats compared with control rats. Glial fibrillary acidic protein (GFAP) and interleukin‐1β (IL‐1β) expression levels in the DRG were increased in DM rats. Upregulation of GFAP is a marker of SGC activation. Targeting the P2Y12 receptor by short hairpin RNA (shRNA) decreased the upregulated expression of P2Y12 mRNA and protein, coexpression of P2Y12 and GFAP, the expression of GFAP, IL‐1β, and tumor necrosis factor‐receptor 1?in the DRG of DM rats, and relieved mechanical and thermal hyperalgesia in DM rats. After treatment with the P2Y12 receptor shRNA, the enhancing integrated OPTICAL density (IOD) ratios of p‐P38 MAPK to P38 mitogen activated protein kinase (MAPK) in the DM rats treated with P2Y12 shRNA were significantly lower than that in the untreated DM rats. Therefore, P2Y12 shRNA treatment decreased SGC activation to relieve mechanical and thermal hyperalgesia in DM rats.
机译：糖尿病神经性疼痛是一种常见的并发症2型糖尿病(DM)。卫星胶质细胞(国网公司)在背根神经节(DRG)在神经性中起着至关重要的作用通过释放促炎的疼痛细胞因子。国网公司打钻。调查的P2Y12受体的作用在糖尿病神经性病变疼痛。神经性疼痛增加机械和热在2型痛觉过敏吗?表明P2Y12的表达水平信使核糖核酸(mRNA)和蛋白质在DRG国网公司增加在DM模型大鼠与控制老鼠。地理地理白介素1β(IL 1β)的表达水平在DM大鼠DRG增加。GFAP是国网公司激活的标志。P2Y12受体通过短发卡RNA(成分)减少了调节P2Y12的表情信使rna和蛋白质,coexpression P2Y12和GFAP的GFAP的表达,高1β和肿瘤坏死地理因子受体1 ?机械和热痛觉过敏DM大鼠。成分,增强集成光密度(IOD)的比率p量P38 MAPK P38有丝分裂原激活蛋白激酶(MAPK) DM大鼠对待P2Y12成分显著降低比未经处理的DM大鼠。P2Y12 shRNA治疗减少了国网公司激活减轻机械和热痛觉过敏在DM老鼠。

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