...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Cellular Physiology >Chick derived induced pluripotent stem cells by the poly‐cistronic transposon with enhanced transcriptional activity
【24h】

Chick derived induced pluripotent stem cells by the poly‐cistronic transposon with enhanced transcriptional activity

机译：小鸡派生的诱导多能干细胞保利cistronic转座子与增强转录活动

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Induced pluripotent stem (iPS) cell technology lead terminally differentiated cells into the pluripotent stem cells through the expression of defined reprogramming factors. Although, iPS cells have been established in a number of mammalian species, including mouse, human, and monkey, studies on iPS cells in avian species are still very limited. To establish chick iPS cells, six factors were used within the poly‐cistronic reprogramming vector (PB‐R6F), containing M3O ( MyoD derived transactivation domain fused with Oct3/4 ), Sox2 , Klf4 , c‐Myc , Lin28 , and Nanog . The PB‐R6F derived iPS cells were alkaline‐phosphatase and SSEA‐1 positive, which are markers of pluripotency. Elevated levels of endogenous Oct3/4 and Nanog genes were detected in the established iPS cells, suggesting the activation of the FGF signaling pathway is critical for the pluripotent status. Histological analysis of teratoma revealed that the established chick iPS cells have differentiation ability into three‐germ‐layer derived tissues. This is the first report of establishment of avian derived iPS cells with a single poly‐cistronic transposon based expression system. The establishment of avian derived iPS cells could contribute to the genetic conservation and modification of avian species.
机译：诱导多能干细胞(iPS)电池技术铅进入终末分化细胞多能干细胞的表达重组因子定义。已经建立了细胞的数量哺乳动物物种,包括小鼠、人类和猴子,研究鸟类物种的“诱导多能性”细胞仍然非常有限。六个因素中使用聚cistronic重组载体(PB必经R6F),包含M3O (MyoD派生transactivation域融合Oct3/4), Sox2 Klf4 c高Myc Lin28, Nanog． 碱性磷酸酶和SSEA高1积极多能性的标志。内源性Oct3/4和Nanog基因被检测到建立了“诱导多能性”细胞,表明的激活FGF信号通路多能性状态的关键。畸胎瘤显示分析建立了小鸡“诱导多能性”细胞分化能力分为三个必经胚芽层派生的组织。这是第一次报告的建立禽流感派生与一个“诱导多能性”细胞基于聚量cistronic转座子表达式系统。细胞可能导致遗传鸟类物种的保护和改造。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号