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首页> 外文期刊>Journal of Cellular Physiology >Lipid composition of membrane microdomains isolated detergent‐free from PUFA supplemented RAW264.7 macrophages
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Lipid composition of membrane microdomains isolated detergent‐free from PUFA supplemented RAW264.7 macrophages

机译：脂质膜microdomains的成分孤立的洗涤剂免费从PUFA补充RAW264.7巨噬细胞

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摘要

Profound alterations in the lipid profile of raft and non‐raft plasma membrane microdomains were found when RAW264.7 macrophages were supplemented with polyunsaturated fatty acids (PUFAs) in physiologically relevant concentrations. For the first time lipids in the detergent‐free isolated membrane domains of phagocytic immune cells were characterized by mass spectrometry. The extent of remodeling of the membrane lipids differed with different n3 and n6 PUFA supplements. The mildest effects were detected for α‐linolenic acid (LNA) and linoleic acid (LA), the C18 precursors of the n3 and n6 families, respectively. When the effects of highly unsaturated PUFAs were compared, eicosapentaenoic acid (EPA) caused more extensive restructuring of membrane lipids than docosahexaenoic acid (DHA) or arachidonic acid (AA). The supplements altered the lipid species composition of both the raft and non‐raft membrane fractions. The rafts containing elevated proportions of highly unsaturated lipid species may relocate sterically incompatible lipids and proteins originally belonging to this microdomain. Such effect was evident for sphingomyelin, which favored non‐rafts instead of rafts after EPA supplementation. The current work suggests that the different functional consequences found previously when supplementing macrophages with either EPA or DHA have their origin in the different effects of these PUFAs on membrane architecture.
机译：脂质筏的深刻变化和非筏质膜microdomains发现当RAW264.7巨噬细胞补充多不饱和脂肪酸(欧米伽)生理浓度相关。第一次脂质在洗涤剂免费孤立噬菌作用的免疫细胞的膜域质谱的特征。重塑膜的脂质不同不同的n3和n6 PUFA补充剂。地理影响被检测到α亚麻酸(放大器)和亚油酸(LA),使用C18前兆的分别n3和n6家庭。高度不饱和的影响欧相比,二十碳五烯酸(EPA)引起更多广泛的重组膜脂质比二十二碳六烯酸(DHA)和花生四烯酸(AA)。筏的成分和非筏膜分数。比例的高度不饱和脂类物种可能搬迁sterically不兼容的脂质和蛋白质最初属于这microdomain。鞘磷脂,支持非木筏代替EPA补充后木筏。表明不同的功能结果发现以前当补充巨噬细胞与EPA和DHA的起源这些欧米伽的不同的效果膜结构。

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