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首页> 外文期刊>Journal of Cellular Physiology >FHOD1 regulates cytoplasmic actin‐based spindle migration for mouse oocyte asymmetric cell division
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FHOD1 regulates cytoplasmic actin‐based spindle migration for mouse oocyte asymmetric cell division

机译：FHOD1调节细胞质肌动蛋白量主轴细胞迁移对小鼠卵母细胞不对称部门

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摘要

FHOD1 is a member of Diaphanous‐related formins (DRFs) which belongs to the Formin family. Previous studies have shown that the DFRs might affect several cellular functions such as morphogenesis, cytokinesis, cell polarity, and embryonic differentiation. However, there is no evidence showing the functions of FHOD1 during oocyte meiosis. This study is aimed at exploring the roles of FHOD1 during the mammalian oocyte maturation. Immunofluorescent staining showed that FHOD1 was restricted to the nucleus in germinal vesicle (GV) stage of the oocytes, after the GV breakdown FHOD1 was primarily located at two poles of the spindle at both metaphases I and II stages. Knockdown of FHOD1 by siRNA injection did not affect polar body extrusion but generated the large polar bodies. In addition, we observed the spindle migration failure in metaphase I oocytes, with a large number of meiotic spindles anchoring in the center of cytoplasm. The expression level of cytoplasmic actin but not cortex actin was significantly reduced, indicating that FHOD1 regulates cytoplasmic actin distribution for the spindle movement. Furthermore, we found that the disruption of ROCK (the Rho‐dependent protein kinase) with inhibitor Y‐27632 caused the decreased FHOD1 protein expression. Therefore, our data indicate that FHOD1 is regulated by ROCK for cytoplasm actin assembly and spindle migration during mouse oocyte meiosis.
机译：FHOD1甲酸精一个精致的成员相关(DRFs)属于甲酸精的家庭。先前的研究已经表明,dfr可能等细胞功能的影响形态发生、胞质分裂、细胞极性,胚胎的分化。证据显示期间FHOD1功能卵母细胞减数分裂。在哺乳动物卵母细胞FHOD1的角色成熟。FHOD1仅限于细胞核胚泡阶段的卵母细胞(问),之后全球之声分解FHOD1主要位于两个在中期我和纺锤体的两极第二阶段。并不影响极体挤压但是生成的呢大极性的身体。在中期我主轴迁移失败卵母细胞,大量的减数分裂纺锤波锚定在细胞质的中心。细胞质肌动蛋白的表达水平,但不是皮质肌动蛋白明显减少,表明FHOD1调节细胞质肌动蛋白分布轴运动。此外,我们发现,岩石的破坏(ρ检测相关的蛋白激酶)抑制剂Y量27632 FHOD1减少蛋白质引起的表达式。FHOD1是细胞质中肌动蛋白受岩石装配和主轴在鼠标迁移卵母细胞减数分裂。

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