...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Cellular Physiology >Application of a novel bioreactor for in vivo engineering of pancreas tissue
【24h】

Application of a novel bioreactor for in vivo engineering of pancreas tissue

机译：应用体内的一种新型生物反应器胰腺组织的工程

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Type 1 diabetes is characterized by autoimmune destruction of pancreatic cells. Organ transplantation is an acceptable treatment for native organ failure. However, it is associated with several problems due to a number of reasons, such as the lack of appropriate donors and immunosuppression. In our present study, a novel model is presented for in vivo recellularization of acellular pancreas by implanting between the host pancreas and the adjacent omental flap. In this study, the pancreases were harvested and cannulated via the common bile duct and then, the scaffolds were acellularized by a detergent‐based protocol. After that, the abdomens of 35 rats were opened and the spleen was extracted with the adjacent omentum, and placed outside the abdomen. The acellularized scaffold was stretched over the host pancreas and the omentum was wrapped around it to make a sandwich‐like structure, which was then fixed with Chromic Sutures 6–0 and marked with Prolene 4–0 on four sides. All samples were biopsied at 14, 30, 60, 90, and 120 days post‐transplantation. The result showed marked recellularization of acellularized pancreas with visible neovascularization and neoβ‐cells with minimal inflammatory response. This study provides a new approach to produces a normal‐like pancreas by allograft transplantation for pancreas tissue engineering. We observed that in vivo transplantation of acellularized pancreas can promote recellularization, proliferation, and differentiation by blood circulation. These findings support that in vivo studies can contribute to finding faster solutions for the treatment of diabetes.
机译：1型糖尿病的特点是自身免疫性胰腺细胞的破坏。移植是一个可接受的治疗本机器官衰竭。有几个问题由于很多原因,比如缺乏适当的捐赠者和免疫抑制。为体内recellularization模型之间的非细胞的胰腺移植主机胰腺和邻网膜的皮瓣。这项研究中,收获和胰腺通过共同的胆管插管,然后支架是由一个洗涤剂acellularized基础协议。开了,脾脏中提取了吗相邻的网膜,放置在腹部。acellularized脚手架被拉伸了主机胰腺和网膜包裹它使一个三明治结构,然后用铬缝合线固定6 - 0和标记四个方面与缝线4 - 0。活检,14岁,30岁,60岁,90年,120天后移植。recellularization acellularized胰腺可见新血管形成和neoβ细胞最小的炎症反应。提供了一种新的方法来产生一个正常的类同种异体移植物移植的胰腺胰腺组织工程。体内acellularized胰腺移植能促进recellularization、扩散和分化的血液循环。发现,体内的研究可以支持为寻找更快的解决方案作出贡献治疗糖尿病。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号