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首页> 外文期刊>Journal of Cellular Physiology >miR‐542‐3p prevents ovariectomy‐induced osteoporosis in rats via targeting SFRP1
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miR‐542‐3p prevents ovariectomy‐induced osteoporosis in rats via targeting SFRP1

机译：miR量542量3 p防止卵巢切除术的诱导通过针对SFRP1老鼠的骨质疏松症

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摘要

Secreted frizzled‐related protein‐1 (SFRP1) is a negative regulatory molecule of the WNT signaling pathway and serves as a therapeutic target for bone formation in osteoporosis. In this study, we first established an ovariectomized (OVX) rat model to simulate postmenopausal osteoporosis and found significant changes in miR‐542‐3p and sFRP1 expression by RNA sequencing and qRT‐PCR. In addition, there was a significant negative correlation between miR‐542‐3p and sFRP1 mRNA levels in postmenopausal women with osteoporosis. We found that miR‐542‐3p inhibited the expression of sFRP1 mRNA by luciferase reporter assay. When the miR‐542‐3p binding site in sFRP1 3'UTR was deleted, it did not affect its expression. Western blot results showed that miR‐542‐3p inhibited the expression of SFRP1 protein. The expression of SFRP1 was significantly increased in osteoblast‐induced mesenchymal stem cells (MSC), whereas the expression of miR‐542‐3p was significantly decreased. And miR‐542‐3p transfected MSCs showed a significant increase in osteoblast‐specific marker expression, indicating that miR‐542‐3p is necessary for MSC differentiation. Inhibition of miR‐542‐3p reduced bone formation, confirmed miR‐542‐3p play a role in bone formation in vivo. In general, these data suggest that miR‐542‐3p play an important role in bone formation via inhibiting SFRP1 expression and inducing osteoblast differentiation.
机译：卷曲的分泌量相关蛋白1 (SFRP1)WNT信号的负调控分子途径和作为治疗目标在骨质疏松症骨形成。首先建立了一个切除卵巢的老鼠(OVX)绝经后骨质疏松症和模型来模拟发现重大变化在米尔542量3 p和sFRP1表达式由RNA和qRT PCR测序。另外,有一个重大的负面相关性miR量542的3 p和sFRP1 mRNA水平在绝经后妇女骨质疏松症。我们发现miR量542 3 p抑制表达式荧光素酶sFRP1 mRNA的记者分析。miR量542检测sFRP1 3 'utr 3 p结合位点删除,不影响其表达。免疫印迹结果表明,miR量542 3 p抑制SFRP1蛋白的表达。表达SFRP1显著增加在成骨细胞诱导间充质干细胞(MSC),而地理miR量542 3 p的表达大大降低了。转染msc有显著提高成骨细胞表达地理标志,指示米尔量542 3 p对于MSC是必要的分化。骨形成,证实miR量542 3 p扮演一个角色在体内骨形成。表明miR量542 3 p中扮演重要角色通过抑制骨形成SFRP1表达式和诱导成骨细胞分化。

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