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首页> 外文期刊>Archiv fur Lebensmittelhygiene >Epidemiologic survey of Clostridium perfringens isolates from foodpoisoning outbreaks by protein fingerprinting and pulse-field gelelectrophoresis [German]
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Epidemiologic survey of Clostridium perfringens isolates from foodpoisoning outbreaks by protein fingerprinting and pulse-field gelelectrophoresis [German]

机译：通过蛋白指纹图谱和脉冲场凝胶电泳从食物中毒暴发事件中产气荚膜梭状芽孢杆菌的流行病学调查[德国]

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摘要

A retrospective study of well characterized isolates of Clostridium perfringens strains from 10 food poisoning outbreaks was performed in order to determine epidemiological links and to evaluate molecular methods. The strains were collected over a period of nearly one decade (1984 to 1991) in south-east Germany. In total 35 strains, 12 of them isolated from food and 23 from patients, which consumed the respective food, have been investigated. Methods applied were the analysis of the total soluble cytoplasmatic proteins by proteinfingerprinting and pulsed-field gel electrophoresis (PFGE) with four different macro-restrictionenzymes (Smal, Kspl, Apal, Sa/l). PFGE showed with all enzymes similar results. in nine outbreaks PFGE was able to confirm, that the food and the patients isolates were identical. The enzyme Sail was most cost effective. Protein analysis could be interpreted only with difficulties and was not as reliable as PFGE. The methods under investigation, especially PFGE, proved to be useful for science-based examinations of clostridia in food-borne diseases in man. For routine examinations they are of minor interest because they are more expensive and laborious than conventional methods.
机译：为了确定流行病学联系并评估分子方法，对10例食物中毒爆发的产气荚膜梭状芽胞杆菌菌株进行了特征明确的回顾性研究。这些菌株是在德国东南部近十年（1984年至1991年）内收集的。总共对35种菌株进行了调查，其中12种是从食物中分离出来的，23种是从食用相应食物的患者中分离出来的。应用的方法是通过蛋白质指纹图谱和具有四种不同的宏观限制性酶（Smal，Kspl，Apal，Sa / l）的脉冲场凝胶电泳（PFGE）分析总可溶性胞质蛋白。 PFGE对所有酶均显示相似的结果。在9次疫情暴发中，PFGE能够确认食物和患者分离株是相同的。酵素帆是最有效的。蛋白质分析只能有困难的解释，并且不如PFGE可靠。事实证明，所研究的方法，尤其是PFGE，对于基于科学的人食源性疾病梭状芽胞杆菌检查非常有用。对于常规检查，它们没有什么意义，因为它们比常规方法昂贵且费力。

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