首页> 外文期刊>Archives of Andrology: An International Journal >Maturation-related changes in distribution of lectin receptors in baboon (Papio anubis) spermatozoa during epididymal maturation.
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Maturation-related changes in distribution of lectin receptors in baboon (Papio anubis) spermatozoa during epididymal maturation.

机译:附睾成熟过程中狒狒(Papio anubis)精子中凝集素受体分布的成熟相关变化。

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摘要

Changes in distribution of surface glycoproteins in baboon sperm were studied by lectin blotting techniques. In baboon, several changes in sperm surface occur during epididymal passage. These changes include increased staining of band that was observed with the WGA binding glycoproteins of 140, 80, 52, and 46 kDa; Con A bands of 66, 37, and 26 kDa; and the PNA binding glycoprotein of 114 kDa. A second change was the loss of preexisting band that was observed with RCA binding glycoproteins of 120, 80, 58, 53, 46, and 37 kDa; and the Con A band of 80 kDa. A final change was noted with Con A binding in which six bands of 8, 9, 12, 14, 18, and 21 kDa were added as the sperm matured through the cauda epididymis. These findings present new information on the changes in distribution of surface glycoproteins in baboon sperm during epididymal passage. There was some reorganization of the molecular structure of the sperm during epididymal maturation.
机译:通过凝集素印迹技术研究了狒狒精子中表面糖蛋白分布的变化。在狒狒中,附睾通过过程中精子表面发生一些变化。这些变化包括用140、80、52和46 kDa的WGA结合糖蛋白观察到的条带染色增加; 66、37和26 kDa的Con A带; PNA结合糖蛋白为114 kDa。第二个变化是使用RCA结合糖蛋白120、80、58、53、46和37 kDa观察到的先前存在的条带丢失。和80 kDa的Con A谱带通过Con A结合注意到了最终变化,其中随着通过附睾的精子成熟,添加了8、9、12、14、18和21 kDa的六个带。这些发现为附睾传代过程中狒狒精子表面糖蛋白分布的变化提供了新的信息。附睾成熟过程中精子的分子结构发生了一些重组。

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