Dual priming oligonucleotide-based multiplex PCR analysis for detection of BRAFV600E mutation in FNAB samples of thyroid nodules in BRAFV600E mutation-prevalent area.

Kwak JY; Kim EK; Kim JKHan JHHong SWPark TSChoi JR

首页> 外文期刊>Head and neck: Journal for the sciences and specialities of the head and neck >Dual priming oligonucleotide-based multiplex PCR analysis for detection of BRAFV600E mutation in FNAB samples of thyroid nodules in BRAFV600E mutation-prevalent area.

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Dual priming oligonucleotide-based multiplex PCR analysis for detection of BRAFV600E mutation in FNAB samples of thyroid nodules in BRAFV600E mutation-prevalent area.

机译：双启动oligonucleotide-based多重PCR分析BRAFV600E突变的检测FNAB BRAFV600E甲状腺结节的样品mutation-prevalent区域。

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BACKGROUND: To evaluate the diagnostic value of dual priming oligonucleotide (DPO)-based multiplex polymerase chain reaction (PCR) for the detection of BRAF(V600E) mutations in ultrasound-guided fine-needle aspiration biopsy (US-FNAB) of thyroid nodules. METHODS: Our institutional review board approved this retrospective study, and informed consent was not required from patients. The 130 patients underwent US-FNAB to evaluate BRAF status in thyroid nodules. In FNAB washouts, DPO-based multiplex PCR, direct DNA sequencing, and PCR-restriction fragment length polymorphism (RFLP) were used to detect BRAF(V600E). The diagnostic performance of these methods was calculated. We compared cytologic results by BRAF status. RESULTS: Diagnostic accuracy and sensitivity were highest when screening with DPO-based multiplex PCR. BRAF(V600E) positivity was a useful marker at thyroid nodules with "suspicious for papillary thyroid carcinoma" or "inadequate" cytological result. CONCLUSIONS: DPO-based multiplex PCR may be an alternative to direct DNA sequencing because of its high sensitivity, high accuracy, and simplicity. BRAF(V600E) may be a useful additional diagnostic marker in BRAF(V600E)-prevalent areas.

机译：背景:评估的诊断价值双启动寡核苷酸(数的多重聚合酶链反应(PCR)检测BRAF V600E突变超声引导下细针穿刺活检(US-FNAB)甲状腺结节。机构审查委员会批准回顾性研究,知情同意需要从病人。接受US-FNAB BRAF状态进行评估甲状腺结节。多重PCR、DNA直接测序和PCR-restriction片段长度多态性(RFLP)是用于检测BRAF V600E。这些方法的诊断性能计算。的地位。当筛选与灵敏度最高DPO-based多重PCR。是一个有用的标记在甲状腺结节乳头状甲状腺癌“可疑”“不足”细胞学结果。DPO-based多重PCR可能是另一种选择因为其高直接DNA测序灵敏度、精度高和简单。BRAF V600E)可能是一个有用的额外的诊断标记在BRAF V600E)盛行的地区。

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来源
《Head and neck: Journal for the sciences and specialities of the head and neck》 |2010年第4期|490-498|共9页
作者
Kwak JY; Kim EK; Kim JKHan JHHong SWPark TSChoi JR;
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作者单位

Department of Radiology, Research Institute of Radiological Science, Yonsei University College of Medicine, Seoul, Korea.;

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正文语种英语
中图分类头部及神经外科学;
关键词
Thyroid Nodule; Multiplex Polymerase Chain Reaction; Determination, DNA Sequencepcr analysisFine-Needle Aspiration Biopsy;

机译：甲状腺结节;多重聚合酶链式反应,测定DNA SequencepcranalysisFine-Needle针吸活组织检查;

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1. Correlation of the BRAFV600E and RAS mutations with clinicopathological characteristics of papillary thyroid cancer using TCGA database analysis [J] . Yue Zhao, Bing-Jie Wu, Duo-Duo Wang, 海南医科大学学报（英文版） . 2019,第001期
2. Correlation of the BRAFV600E and RAS mutations with clinicopathological characteristics of papillary thyroid cancer using TCGA database analysis [J] . Yue Zhao, Bing-Jie Wu, Duo-Duo Wang, 海南医科大学学报(英文版) . 2019,第001期
3. BRAFV600E vs. TIRADS in predicting papillary thyroid cancers in Bethesda system Ⅰ,Ⅲ, and Ⅴ nodules [J] . Ya Wu, Ting Xu, Xingyue Cao, 癌症生物学与医学：英文版 . 2019,第001期
4. Dual priming oligonucleotide-based multiplex PCR analysis for detection of BRAFV600E mutation in FNAB samples of thyroid nodules in BRAFV600E mutation-prevalent area. [J] . Kwak JY, Kim EK, Kim JK, Head and neck: Journal for the sciences and specialities of the head and neck . 2010,第4期

机译：基于双引物寡核苷酸的多重PCR分析用于检测BRAFV600E突变流行区域的甲状腺结节FNAB样品中的BRAFV600E突变。

Dual priming oligonucleotide-based multiplex PCR analysis for detection of BRAFV600E mutation in FNAB samples of thyroid nodules in BRAFV600E mutation-prevalent area.

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